SubtiBank SubtiBank
Version comparison:

Sat May 21 2016 12:50:17 GMT+0200 (CEST)2025-06-01 07:44:02

locus

BSU32000

BSU_32000

outlinks

bsu

BSU32000

BSU_32000

[SW|Categories] containing this gene/protein

[SW|acquisition of iron], [SW|iron metabolism]

Gene

Coordinates on the chromosome (coding sequence)

3,291,511 -> 3,292,296

The protein

Catalyzed reaction/ biological activity

2,3-dihydro-2,3-dihydroxybenzoate NAD = 2,3-dihydroxybenzoate NADH (according to Swiss-Prot)

(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + NAD+ --> 2,3-dihydroxybenzoate + H+ + NADH (according to UniProt)

The protein

Protein family

short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

[SW|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

Expression and Regulation

Operon

''[[protein|dhbA]]-[[protein|dhbC]]-[[protein|dhbE]]-[[protein|dhbB]]-[[protein|dhbF]]'' [Pubmed|8921902]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|8550523]

Expression and Regulation

Regulation

induced by iron starvation ([[protein|Fur]]) [Pubmed|12354229]

Expression and Regulation

Regulatory mechanism

[[protein|Fur]]: transcription repression [Pubmed|12354229]

[[protein|AbrB]]: transcription repression [Pubmed|20817675]

Expression and Regulation

Additional information

the ''[[protein|dhbA]]-[[protein|dhbC]]-[[protein|dhbE]]-[[protein|dhbB]]-[[protein|dhbF]]'' operon is strongly unregulated in a ''[SW|kre]'' mutant [Pubmed|26110430]

References

8224884, 10400588, 11112781, 11790741, 12354229, 8921902, 8550523, 20525796, 20817675, 21815947, 24223955, 26110430

8224884, 10400588, 11112781, 11790741, 12354229, 8921902, 8550523, 20525796, 20817675, 21815947, 24223955, 26110430, 29133393, 29914988, 16790929, 31113899, 31420537

proteinLength

261

geneLength

786

Gene

Coordinates

3,291,511 3,292,296

Gene

Phenotypes of a mutant

defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium [pubmed|31420537]

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|2FWM] (from E. coli, 42% identity) [pubmed|16790929]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE32000 ([[gene|dhbA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATCAATTCCTTTCT, downstream forward: _UP4_TAACTGAATTTAAAGGAGGT

BKK32000 ([[gene|dhbA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATCAATTCCTTTCT, downstream forward: _UP4_TAACTGAATTTAAAGGAGGT