2019-02-19 19:04:322018-12-07 15:39:06
geneLength
1950
1947
Gene
Coordinates
842,047 843,996
842,047 → 843,996
Gene
Phenotypes of a mutant
induction of [[protein|SigM]] and [[protein|SigX]] activities [Pubmed|23103977]
reduced [[protein|SigD]]-dependent expression of ''[[gene|lytF]]'' [Pubmed|25288647]
extended lag phase [pubmed|29114240]
reduced colony size with accumulation of dead cells in the colonies [pubmed|29114240]
suppression of the growth and morphology defects of a [[gene|glmR]] mutant [pubmed|30478337]
induction of [[protein|SigM]] and [[protein|SigX]] activities [Pubmed|23103977]
reduced [[protein|SigD]]-dependent expression of ''[[gene|lytF]]'' [Pubmed|25288647]
extended lag phase [pubmed|29114240]
reduced colony size with accumulation of dead cells in the colonies [pubmed|29114240]
The protein
Modification
phosphorylated on Thr-297 [Pubmed|19229300]
can be phosphorylated by [[protein|PrkC]] in vitro [pubmed|30478337]
phosphorylated on Thr-297 [Pubmed|19229300]
Biological materials
Mutant
MGNA-C256 (yflE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2254 NBRP B. subtilis, Japan]
GP1389 ''ltaS::aphA3'', available in [SW|Jrg Stlke]'s lab
GP1396 ''ltaS::tet'', available in [SW|Jrg Stlke]'s lab
BKE07710 ([[gene|ltaS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTACACTCCTTTTTT, downstream forward: _UP4_TAAGAAAAAGCGGAGAGGTT
BKK07710 ([[gene|ltaS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTACACTCCTTTTTT, downstream forward: _UP4_TAAGAAAAAGCGGAGAGGTT
MGNA-C256 (yflE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2254 NBRP B. subtilis, Japan]
GP1389 ''ltaS::aphA3'', available in [SW|Jörg Stülke]'s lab
GP1396 ''ltaS::tet'', available in [SW|Jörg Stülke]'s lab
BKE07710 (Δ[[gene|ltaS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTACACTCCTTTTTT, downstream forward: _UP4_TAAGAAAAAGCGGAGAGGTT
BKK07710 (Δ[[gene|ltaS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTACACTCCTTTTTT, downstream forward: _UP4_TAAGAAAAAGCGGAGAGGTT
References
Original publications