2019-02-19 19:04:292025-05-12 05:12:59
locus
BSU16120
BSU_16120
essential
yes
quasi
outlinks
bsu
BSU16120
BSU_16120
Gene
Phenotypes of a mutant
essential [Pubmed|25092907], non-essential according to [Pubmed|28189581], but only one resistance cassette was possible
essential [Pubmed|25092907]
non-essential according to [Pubmed|28189581], but only one resistance cassette was possible
essential, deletion of [[gene|topA]] results in the immediate acquisition of suppressor mutations that result in overexpression of [[protein|ParE]]-[[protein|ParC]] [pubmed|30957856]
The protein
Catalyzed reaction/ biological activity
relaxation of negatively supercoiled DNA
relaxation of negatively supercoiled DNA
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to UniProt)
The protein
Protein family
topo IA family (according to Swiss-Prot)
Type IA topoisomerase family (together with [[protein|TopB]])(according to UniProt)
Biological materials
Mutant
BKK16120 ([[gene|topA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCCCCCTCAAGA, downstream forward: _UP4_TAGCGGTGAGCAGGGTCTTG
BKK16120 (Δ[[gene|topA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCCCCCTCAAGA, downstream forward: _UP4_TAGCGGTGAGCAGGGTCTTG
GP1963 (Δ[[gene|topA]]::aphA3 (citB-alsT)16) [pubmed|30957856]
Biological materials
Expression vectors
pGP2060: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jrg Stlke]'s lab
pGP2060: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
References
Original publications