Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-09 08:53:44
description
membrane pseudokinase-like protein, part of the type VII protein secretion system YukD-YukC-YukB-YueB-YueC
membrane pseudokinase-like protein, part of the type VII protein secretion system [[protein|YukD]]-[[protein|YukC]]-[[protein|YukB]]-[[protein|YueB]]-[[protein|YueC]]
locus
BSU31890
BSU_31890
function
export of YukE
export of [[protein|YukE]]
ec
outlinks
bsu
BSU31890
BSU_31890
aminos
MSGEQKSYLENQLEAVAEKTDAGYTFTFQREKIKLLDGLEANVIKDINPFFHKEIDVTDDEVIITIQPPSSYKAFRFMKAKDKKSKWQFAYQLVQAVQQHNLSRLNLIVAPENIVFDKGLTPYFLHYGVKESIPPYERDEERVWQELKAAAALAVDGAFAFEDYLKFNETLTFSAEAKAILDAESYDDLLELIQTHIDELEAKAKTYIHIPRKKWNIQRYIGLGLIVLLVPALIYSMYALFFAQPKHQAIVDSNRAFLNKQYSEVISTLSKYDAESLPESVQYQLATSYVEVENLGSAKTKNIENNLVTLQSDPQHFLYWIDYGRGEYKEAISIGRKLEYNDYIYFALAKYKQQLLSEDTNDEDIQKELDSVNSELEKAQKERQENKQSNSETSLVDTSEEQTQTDEEKQAEEKAAEEKAAAEEKAKKEEQKEKEDEKKETEKKDEKKDDK
[SW|Categories] containing this gene/protein
[SW|protein secretion], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
3,274,462 -> 3,275,817
The protein
[SW|Localization]
membrane (according to SwissProt) [Pubmed|23861817]
cell membrane (according to UniProt) [Pubmed|23861817]
Expression and Regulation
Operon
''[[protein|yukE]]-[[protein|yukD]]-[[protein|yukC]]-[[protein|yukB]]-[[protein|yueB]]-[[protein|yueC]]'' [Pubmed|22383849,15576783]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|22383849]
Expression and Regulation
Regulation
not expressed in the lab strain 168, due to lack of [[protein|DegU]]-P [Pubmed|23861817]
expressed in the stationary phase [Pubmed|23861817]
Expression and Regulation
Regulatory mechanism
[[protein|DegU]]-P: transcription activation [Pubmed|23861817]
References
Original publications
proteinLength
451
geneLength
1356
Gene
Coordinates
3,274,462 3,275,817
The protein
Protein family
EssB family (single member, according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|4ANO] (from Geobacillus thermodenitrificans, corresponds to the cytoplasmic part, aa 2 ... 218, 44% identity) [pubmed|23499020]
[PDB|2YNQ] (from Geobacillus thermodenitrificans, corresponds to the extracellular part, aa 243 ... 384, 40% identity) [pubmed|23499020]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A626 (yukC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/626 NBRP B. subtilis, Japan]
BKE31890 ([[gene|yukC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCTGACATTCAACCAGATC, downstream forward: _UP4_TAACGTGAGACATGAAGCGC
BKK31890 ([[gene|yukC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCTGACATTCAACCAGATC, downstream forward: _UP4_TAACGTGAGACATGAAGCGC