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Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-09 08:53:44

description

membrane pseudokinase-like protein, part of the type VII protein secretion system YukD-YukC-YukB-YueB-YueC

membrane pseudokinase-like protein, part of the type VII protein secretion system [[protein|YukD]]-[[protein|YukC]]-[[protein|YukB]]-[[protein|YueB]]-[[protein|YueC]]

locus

BSU31890

BSU_31890

function

export of YukE

export of [[protein|YukE]]

ec

outlinks

bsu

BSU31890

BSU_31890

aminos

MSGEQKSYLENQLEAVAEKTDAGYTFTFQREKIKLLDGLEANVIKDINPFFHKEIDVTDDEVIITIQPPSSYKAFRFMKAKDKKSKWQFAYQLVQAVQQHNLSRLNLIVAPENIVFDKGLTPYFLHYGVKESIPPYERDEERVWQELKAAAALAVDGAFAFEDYLKFNETLTFSAEAKAILDAESYDDLLELIQTHIDELEAKAKTYIHIPRKKWNIQRYIGLGLIVLLVPALIYSMYALFFAQPKHQAIVDSNRAFLNKQYSEVISTLSKYDAESLPESVQYQLATSYVEVENLGSAKTKNIENNLVTLQSDPQHFLYWIDYGRGEYKEAISIGRKLEYNDYIYFALAKYKQQLLSEDTNDEDIQKELDSVNSELEKAQKERQENKQSNSETSLVDTSEEQTQTDEEKQAEEKAAEEKAAAEEKAKKEEQKEKEDEKKETEKKDEKKDDK

[SW|Categories] containing this gene/protein

[SW|protein secretion], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

3,274,462 -> 3,275,817

The protein

[SW|Localization]

membrane (according to SwissProt) [Pubmed|23861817]

cell membrane (according to UniProt) [Pubmed|23861817]

Expression and Regulation

Operon

''[[protein|yukE]]-[[protein|yukD]]-[[protein|yukC]]-[[protein|yukB]]-[[protein|yueB]]-[[protein|yueC]]'' [Pubmed|22383849,15576783]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|22383849]

Expression and Regulation

Regulation

not expressed in the lab strain 168, due to lack of [[protein|DegU]]-P [Pubmed|23861817]

expressed in the stationary phase [Pubmed|23861817]

Expression and Regulation

Regulatory mechanism

[[protein|DegU]]-P: transcription activation [Pubmed|23861817]

References

Original publications

15576783, 22383849, 23861817, 24798022

15576783, 22383849, 23861817, 24798022, 23499020

proteinLength

451

geneLength

1356

Gene

Coordinates

3,274,462 3,275,817

The protein

Protein family

EssB family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|4ANO] (from Geobacillus thermodenitrificans, corresponds to the cytoplasmic part, aa 2 ... 218, 44% identity) [pubmed|23499020]

[PDB|2YNQ] (from Geobacillus thermodenitrificans, corresponds to the extracellular part, aa 243 ... 384, 40% identity) [pubmed|23499020]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A626 (yukC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/626 NBRP B. subtilis, Japan]

BKE31890 ([[gene|yukC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCTGACATTCAACCAGATC, downstream forward: _UP4_TAACGTGAGACATGAAGCGC

BKK31890 ([[gene|yukC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCTGACATTCAACCAGATC, downstream forward: _UP4_TAACGTGAGACATGAAGCGC