2017-08-16 12:49:192025-04-15 04:07:46
description
choline [SW|ABC transporter ](ATP-binding protein)
choline and arsenocholine [SW|ABC transporter] (ATP-binding protein)
locus
BSU33730
BSU_33730
geneLength
1143
1146
product
choline [SW|ABC transporter ](ATP-binding protein)
choline and arsenocholine [SW|ABC transporter] (ATP-binding protein)
outlinks
bsu
BSU33730
BSU_33730
Gene
Coordinates
3,462,105 → 3,463,250
3,462,105 3,463,250
The protein
Protein family
[SW|ABC transporter] family (according to Swiss-Prot)
[SW|ABC transporter superfamily] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|OpuAA]], [[protein|OpuCA]]
[[this]]
The protein
[SW|Domains]
2 [SW|CBS domain]s (256-314, 316-374)
2 [SW|CBS domain]s (aa 256-314, aa 316-374) (according to UniProt)
[SW|ABC transporter domain] (aa 2-236) (according to UniProt)
Biological materials
Expression vector
pGP2938 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
References
Original publications
The protein
Catalyzed reaction/ biological activity
uptake of choline and arsenocholine [pubmed|29159878]
Biological materials
Mutant
BKE33730 ([[gene|opuBA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
BKK33730 ([[gene|opuBA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
Biological materials
Expression vectors
pGP2938: (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP3013: (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab