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Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-28 00:51:08

description

choline ABC transporter (ATP-binding protein)

choline and arsenocholine [SW|ABC transporter] (ATP-binding protein)

locus

BSU33730

BSU_33730

product

choline ABC transporter (ATP-binding protein)

choline and arsenocholine [SW|ABC transporter] (ATP-binding protein)

ec

outlinks

bsu

BSU33730

BSU_33730

[SW|Categories] containing this gene/protein

[SW|ABC transporters], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

3,462,105 -> 3,463,250

The protein

Protein family

[SW|ABC transporter] family (according to Swiss-Prot)

[SW|ABC transporter superfamily] (according to UniProt)

The protein

Paralogous protein(s)

[SW|OpuCA]

[[this]]

The protein

[SW|Domains]

2 [SW|CBS domain]s (256-314, 316-374)

2 [SW|CBS domain]s (aa 256-314, aa 316-374) (according to UniProt)

[SW|ABC transporter domain] (aa 2-236) (according to UniProt)

The protein

[SW|Localization]

associated to the membrane (via [SW|OpuBB]-[SW|OpuBD]) [Pubmed|10092453]

associated to the membrane (via [[protein|OpuBB]]-[[protein|OpuBD]]) [Pubmed|10092453]

The protein

[SW|Interactions]

[SW|OpuBA]-([SW|OpuBB]-[SW|OpuBD])-[SW|OpuBC] [Pubmed|10092453]

Expression and Regulation

Operon

''[SW|opuBA]-[SW|opuBB]-[SW|opuBC]-[SW|opuBD]'' [Pubmed|10216873]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|10216873]

Expression and Regulation

Regulation

induced by salt stress [Pubmed|10216873]

induced by choline ([[protein|GbsR]]) [Pubmed|22408163]

Expression and Regulation

Regulatory mechanism

[[protein|GbsR]]: transcription repression [Pubmed|22408163]

[[protein|OpcR]]: transcription repression [Pubmed|23960087]

[[protein|RemA]]: transcription activation [Pubmed|23646920]

References

10092453, 9925583, 10216873, 21296969, 23960087, 22408163, 23646920

proteinLength

381

geneLength

1146

Gene

Coordinates

3,462,105 3,463,250

The protein

Catalyzed reaction/ biological activity

uptake of choline and arsenocholine [pubmed|29159878]

The protein

Structure

[PDB|2IT1] (from Pyrococcus horikoshii, 43% identity)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE33730 ([[gene|opuBA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA

BKK33730 ([[gene|opuBA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA

Biological materials

Expression vectors

pGP2938: (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP3013: (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

References

Reviews

27935846

References

Original publications

10092453, 9925583, 10216873, 21296969, 23960087, 22408163, 23646920, 29159878, 32373088, 32849357