Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-28 00:51:08
description
choline ABC transporter (ATP-binding protein)
choline and arsenocholine [SW|ABC transporter] (ATP-binding protein)
locus
BSU33730
BSU_33730
product
choline ABC transporter (ATP-binding protein)
choline and arsenocholine [SW|ABC transporter] (ATP-binding protein)
ec
outlinks
bsu
BSU33730
BSU_33730
[SW|Categories] containing this gene/protein
[SW|ABC transporters], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
3,462,105 -> 3,463,250
The protein
Protein family
[SW|ABC transporter] family (according to Swiss-Prot)
[SW|ABC transporter superfamily] (according to UniProt)
The protein
Paralogous protein(s)
[SW|OpuCA]
[[this]]
The protein
[SW|Domains]
2 [SW|CBS domain]s (256-314, 316-374)
2 [SW|CBS domain]s (aa 256-314, aa 316-374) (according to UniProt)
[SW|ABC transporter domain] (aa 2-236) (according to UniProt)
The protein
[SW|Localization]
associated to the membrane (via [SW|OpuBB]-[SW|OpuBD]) [Pubmed|10092453]
associated to the membrane (via [[protein|OpuBB]]-[[protein|OpuBD]]) [Pubmed|10092453]
The protein
[SW|Interactions]
[SW|OpuBA]-([SW|OpuBB]-[SW|OpuBD])-[SW|OpuBC] [Pubmed|10092453]
Expression and Regulation
Operon
''[SW|opuBA]-[SW|opuBB]-[SW|opuBC]-[SW|opuBD]'' [Pubmed|10216873]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|10216873]
Expression and Regulation
Regulation
induced by salt stress [Pubmed|10216873]
induced by choline ([[protein|GbsR]]) [Pubmed|22408163]
Expression and Regulation
Regulatory mechanism
[[protein|GbsR]]: transcription repression [Pubmed|22408163]
[[protein|OpcR]]: transcription repression [Pubmed|23960087]
[[protein|RemA]]: transcription activation [Pubmed|23646920]
References
proteinLength
381
geneLength
1146
Gene
Coordinates
3,462,105 3,463,250
The protein
Catalyzed reaction/ biological activity
uptake of choline and arsenocholine [pubmed|29159878]
The protein
Structure
[PDB|2IT1] (from Pyrococcus horikoshii, 43% identity)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE33730 ([[gene|opuBA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
BKK33730 ([[gene|opuBA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
Biological materials
Expression vectors
pGP2938: (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP3013: (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
References
Reviews
References
Original publications