Sun Dec 18 2016 20:27:19 GMT+0100 (CET)2017-02-27 15:02:04
[SW|Categories] containing this gene/protein
[SW|RNAses], [SW|essential genes]
Gene
Phenotypes of a mutant
the mutant is strongly impaired in [SW|sporulation], [SW|genetic competence] and many other phenotypes [Pubmed|23504012]
it is not possible to construct a ''[SW|rnjA] [SW|rny]'' double mutant [Pubmed|23504012]
the mutant is strongly impaired in [SW|sporulation], [SW|genetic competence] and many other phenotypes [Pubmed|23504012]
it is not possible to construct a ''[[gene|rnjA]] [[gene|rny]]'' double mutant [Pubmed|23504012]
The protein
Catalyzed reaction/ biological activity
endonuclease and 5'-3' exonuclease
endonuclease and 5'-3' exonuclease
degrades [[protein|SR5]] and ''[[gene|gapA]]'' RNAs [SW|27449348]
The protein
[SW|Interactions]
part of the [SW|RNA degradosome] [Pubmed|19193632]
[[protein|RnjA]]-[[protein|RnjB]] [Pubmed|19193632,20025672,27708634]
[[protein|RnjA]]-[[protein|PfkA]] [Pubmed|19193632]
[[protein|RnjA]]-[[protein|PnpA]] [Pubmed|19193632], K(D) of the interaction: 250 nM [Pubmed|22198292]
[[protein|RnjA]]-[[protein|Rny]] [Pubmed|19193632,21803996]
[[protein|RnjA]]-[[protein|CshA]] [Pubmed|20572937]
[SW|GapA]-[SW|RnjA] [Pubmed|27449348], about 1% of all [SW|GapA] molecules participate in this interaction [Pubmed|27449348], this interaction is stabilized in the presence of [SW|YkzW] [Pubmed|27449348]
The protein
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
required for ''[[protein|thrS]]'' RNA processing, involved in maturation of the 5’-end of the16S rRNA
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
required for ''[[gene|thrS]]'' RNA processing, involved in maturation of the 5’-end of the16S rRNA
Expression and Regulation
Operon
''[[protein|rpoY]]-[[protein|rnjA]]'' [Pubmed|24187087]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|24187087]
Expression and Regulation
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
translation of [[protein|YkzG]] and [[protein|RnjA]] is coupled, and this coupling is required for efficient expression of [[rnjA|RNase J1]] [PubMed|24187087]
Biological materials
Mutant
GP41 (''rnjA'' under control of p(xyl)), available in [SW|Jörg Stülke]'s lab
SSB342 (''rnjA'' under pspac), cat, available in [SW|Harald Putzer] lab
GP2506 (''[[protein|rnjA]]''::''spc''), available in [SW|Jörg Stülke]'s lab
GP41 (''rnjA'' under control of p(xyl)), available in [SW|Jörg Stülke]'s lab
SSB342 (''rnjA'' under pspac), cat, available in [SW|Harald Putzer] lab
GP2506 (''[[gene|rnjA]]''::''spc''), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vector
for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [SW|Jörg Stülke]'s lab
for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [SW|Jörg Stülke]'s lab
for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [SW|Jörg Stülke]'s lab
for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [SW|Jörg Stülke]'s lab
pGP1441: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
References
Reviews
proteinLength
555
geneLength
1665
The protein
Effectors of protein activity
initeraction with [[protein|GapA]]-[[protein|Sr1]] stimulates activity [Pubmed|27449348]
Expression and Regulation
[[this]]
Biological materials
GFP fusion
GP1694 (in pHJS-105 [Pubmed|26110430]), expression of '' rnjA-sfGFP''::''spc'' under a xylose-inducible promoter in '' B. subtilis'', available in [SW|Jörg Stülke]'s lab [Pubmed|27708634]
GP1722 (in [SW|pBP43]), expression of '' rnjA-GFP''::''spc'' under the native promoter, available in [SW|Jörg Stülke]'s lab [Pubmed|27708634]