SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:54:15 GMT+0200 (CEST)2025-05-10 06:36:08

locus

BSU15720

BSU_15720

outlinks

bsu

BSU15720

BSU_15720

[SW|Categories] containing this gene/protein

[SW|translation], [SW|protein modification]

Gene

Coordinates on the chromosome (coding sequence)

1,646,512 -> 1,646,994

The protein

Catalyzed reaction/ biological activity

Formyl-L-methionyl peptide H2O = formate methionyl peptide (according to Swiss-Prot)

H2O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)

The protein

Protein family

polypeptide deformylase family (according to Swiss-Prot)

polypeptide deformylase family (with [[protein|DefB]], according to UniProt)

The protein

Paralogous protein(s)

[[protein|DefB]]

[[this]]

The protein

[SW|Interactions]

[[protein|DefA]]-[[protein|RplV]] [Pubmed|18288106]

[[protein|Map]] competes with peptide deformylase ([[protein|DefA]]), the first enzyme to act on nascent chains, for binding sites at the ribosomal tunnel exit [Pubmed|23770820]

Expression and Regulation

Operon

''[[protein|defA]]-[[protein|fmt]]-[[protein|yloM]]'' [Pubmed|16964327]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] ''[[protein|defA]]-[[protein|fmt]]-[[protein|yloM]]'' [Pubmed|16964327]

References

11429456, 12627383, 18288106, 16964327, 23770820

11429456, 12627383, 18288106, 16964327, 23770820, 27983482, 16049914

proteinLength

160

geneLength

483

Gene

Coordinates

1,646,512 1,646,994

Gene

Phenotypes of a mutant

a ''[[gene|defA]] [[gene|defB]]'' double mutant is not viable unless the cells acquire suppressor mutations in ''[[gene|fmt]], [[gene|glyA]]'' or ''[[gene|folD]]'' [Pubmed|27983482]

The protein

Structure

[PDB|1WS0] (from B.cereus, 67% identity) [pubmed|16049914]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE15720 ([[gene|defA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG

BKK15720 ([[gene|defA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG