Mon Aug 31 2015 13:54:15 GMT+0200 (CEST)2025-05-10 06:36:08
locus
BSU15720
BSU_15720
outlinks
bsu
BSU15720
BSU_15720
[SW|Categories] containing this gene/protein
[SW|translation], [SW|protein modification]
Gene
Coordinates on the chromosome (coding sequence)
1,646,512 -> 1,646,994
The protein
Catalyzed reaction/ biological activity
Formyl-L-methionyl peptide
H2O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)
The protein
Protein family
polypeptide deformylase family (according to Swiss-Prot)
polypeptide deformylase family (with [[protein|DefB]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|DefB]]
[[this]]
The protein
[SW|Interactions]
[[protein|DefA]]-[[protein|RplV]] [Pubmed|18288106]
[[protein|Map]] competes with peptide deformylase ([[protein|DefA]]), the first enzyme to act on nascent chains, for binding sites at the ribosomal tunnel exit [Pubmed|23770820]
Expression and Regulation
Operon
''[[protein|defA]]-[[protein|fmt]]-[[protein|yloM]]'' [Pubmed|16964327]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] ''[[protein|defA]]-[[protein|fmt]]-[[protein|yloM]]'' [Pubmed|16964327]
References
proteinLength
160
geneLength
483
Gene
Coordinates
1,646,512 1,646,994
Gene
Phenotypes of a mutant
a ''[[gene|defA]] [[gene|defB]]'' double mutant is not viable unless the cells acquire suppressor mutations in ''[[gene|fmt]], [[gene|glyA]]'' or ''[[gene|folD]]'' [Pubmed|27983482]
The protein
Structure
[PDB|1WS0] (from B.cereus, 67% identity) [pubmed|16049914]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE15720 ([[gene|defA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG
BKK15720 ([[gene|defA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG