2018-10-05 19:06:502025-05-27 17:04:47
description
similar to hydroxycarboxylic acid dehydrogenase, may be involved in galacturonate utilization
similar to ureidoglycolate dehydrogenase, may be involved in galacturonate utilization
locus
BSU12320
BSU_12320
geneLength
1011
1014
outlinks
bsu
BSU12320
BSU_12320
Gene
Coordinates
1,303,423 → 1,304,436
1,303,423 1,304,436
The protein
Protein family
LDH2/MDH2 oxidoreductase family (according to Swiss-Prot)
LDH2/MDH2 oxidoreductase family (single member, according to UniProt)
The protein
Structure
[PDB|4H8A] (41% identity)
[PDB|4H8A] (ureidoglycolate dehydrogenase from E. coli, 41% identity) [pubmed|23284870]
The protein
Additional information
The gene is annotated in KEGG as an ortholog of malate dehydrogenase EC 1.1.1.37. but it is marked as “uncharacterized oxidoreductase” (EC 1.1.1.-) in Swiss-Prot. In MetaCyc it is marked as “similar to malate dehydrogenase”. As the paper by Mekjian et al. [Pubmed|9882655] suggests this gene is more likely to be involved in the glucuronate pathway (for which EC 1.1.1.37 is not a member), no literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
The gene is annotated in KEGG as an ortholog of malate dehydrogenase EC 1.1.1.37. but it is marked as uncharacterized oxidoreductase (EC 1.1.1.-) in Swiss-Prot. In MetaCyc it is marked as similar to malate dehydrogenase. As the paper by Mekjian et al. [Pubmed|9882655] suggests this gene is more likely to be involved in the glucuronate pathway (for which EC 1.1.1.37 is not a member), no literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
Biological materials
Mutant
MGNA-A367 (yjmC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/367 NBRP B. subtilis, Japan]
BKE12320 (Δ[[gene|yjmC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCAAGTGCCTCCTTCCT, downstream forward: _UP4_TTCTTAAAAAGCAGGTGAGT
BKK12320 (Δ[[gene|yjmC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCAAGTGCCTCCTTCCT, downstream forward: _UP4_TTCTTAAAAAGCAGGTGAGT
MGNA-A367 (yjmC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/367 NBRP B. subtilis, Japan]
BKE12320 ([[gene|yjmC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCAAGTGCCTCCTTCCT, downstream forward: _UP4_TTCTTAAAAAGCAGGTGAGT
BKK12320 ([[gene|yjmC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCAAGTGCCTCCTTCCT, downstream forward: _UP4_TTCTTAAAAAGCAGGTGAGT
References