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Mon Aug 31 2015 13:57:37 GMT+0200 (CEST)2025-05-16 02:35:37

description

trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBCA of the PTS

beta-glucoside permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIBCA of the [[category|Phosphotransferase system|PTS]], [[category|Trigger enzyme]], control of [[protein|LicT ]]activity

locus

BSU39270

BSU_39270

function

beta-glucoside uptake and phosphorylation,
control of LicT activity

beta-glucoside uptake and phosphorylation, control of [[protein|LicT ]]activity

product

trigger enzyme: beta-glucoside-specific
phosphotransferase system, EIIBCA

beta-glucoside permease of the [SW|phosphotransferase systems|phosphotransferase system]

outlinks

bsu

BSU39270

BSU_39270

syns

sytA

[SW|Categories] containing this gene/protein

[SW|phosphotransferase systems], [SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|trigger enzyme], [SW|membrane proteins], [SW|phosphoproteins]

The protein

Catalyzed reaction/ biological activity

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

The protein

Protein family

[SW|PTS] permease, sucrose permease (Scr) family [Pubmed|10627040]

[[category|Phosphotransferase system|PTS]] permease, sucrose family [Pubmed|10627040]

The protein

[SW|Interactions]

[[protein|BglP]]-[[PtsH|HPr]], [[protein|BglP]]-[[protein|LicT]]

Expression and Regulation

Operon

''[[protein|bglP]]-[[protein|bglH]]-[[protein|yxiE]]'' [Pubmed|7883710]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|7559347]

Expression and Regulation

Regulation

repressed by glucose ([[protein|CcpA]], [[protein|LicT]]) [Pubmed|7559347]

induced by salicin ([[protein|LicT]]) [Pubmed|7883710]

Expression and Regulation

Regulatory mechanism

[[protein|CcpA]]: transcription repression, ([[protein|CcpA]] binding site overlaps -35 region) [Pubmed|7559347]

[[protein|LicT]]-dependent [SW|RNA switch] (antitermination, lack of [[protein|LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[protein|LicT]] to be phosphorylated by [[PtsH| HPr]] [Pubmed|8626332]

Biological materials

Mutant

GP475 (erm), available in the [SW|Stülke] lab

GP475 (erm), available in [SW|Jörg Stülke]'s lab [pubmed|23475962]

BKE39270 ([[gene|bglP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA

BKK39270 ([[gene|bglP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA

Biological materials

Expression vector

pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Stülke] lab,

pGP1300 (expression of ''[[protein|bglP]]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Stülke] lab

Biological materials

lacZ fusion

pGP600 (in [[protein|pAC6]]), available in [SW|Stülke] lab

pGP600 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab

proteinLength

609

geneLength

1830

Gene

Coordinates

4,033,778 4,035,607

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|PTS EIIA domain] type-1 (aa 480-584) (according to UniProt)

[SW|PTS EIIB domain] type-1 (aa 1-86) (according to UniProt)

[SW|PTS EIIC domain] type-1 (aa 103-459) (according to UniProt)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab,

pGP1300 (expression of ''[[gene|bglP]]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

Biological materials

GFP fusion

GP1266, [[gene|bglP]]-cfp, available in [SW|Jörg Stülke]'s lab [pubmed|23475962]