SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-28 06:51:35

locus

BSU22480

BSU_22480

outlinks

bsu

BSU22480

BSU_22480

aminos

MKIALIAHDKKKQDMVQFTTAYRDILKNHDLYATGTTGLKIHEATGLQIERFQSGPLGGDQQIGALIAANALDLVIFLRDPLTAQPHEPDVSALIRLCDVYSIPLATNMGTAEILVRTLDEGVFEFRDLLRGEEPNV

[SW|Categories] containing this gene/protein

[SW|carbon core metabolism]

Gene

Coordinates on the chromosome (coding sequence)

2,358,911 -> 2,359,324

The protein

Catalyzed reaction/ biological activity

Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)

dihydroxyacetone phosphate --> methylglyoxal + phosphate (according to UniProt)

The protein

Protein family

RNase Z family (according to Swiss-Prot)

methylglyoxal synthase family (single member, according to UniProt)

The protein

Effectors of protein activity

non-phosphorylated [[protein|Crh]] interacts with [[protein|MgsA]] to inhibit its activity [Pubmed|21992469]

non-phosphorylated [[protein|Crh]] interacts with [[protein|MgsA]] to inhibit its activity [Pubmed|29514981,21992469]

The protein

Structure

[PDB|1B93] (from ''Escherichia coli'', 50% identity, 73% similarity) [Pubmed|10368300]

[PDB|6F2C] [pubmed|29514981]

The protein

[SW|Interactions]

[[protein|Crh]]-[[protein|MgsA]] (only non-phosphorylated [[protein|Crh]] interacts with [[protein|MgsA]]) [Pubmed|21992469]

Expression and Regulation

Operon

''[[protein|ypjD]]-[[protein|dapB]]-[[protein|mgsA]]-[SW|bshB1]-[[protein|bshA]]-[[protein|cca]]-[[protein|birA]]'' [Pubmed|20308541]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|23894131]

Expression and Regulation

Regulation

induced by diamide stess (thiol depletion) ([[protein|Spx]]) [Pubmed|23894131]

Expression and Regulation

Regulatory mechanism

[[protein|Spx]]: transcription activation [Pubmed|23894131]

Biological materials

Mutant

GP67 (''mgsA''::''tet'') [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab

GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab

MGNA-A440 ([[gene|mgsA]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]

GP67 ([[gene|mgsA]]::''tet'') [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab

GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab

BKE22480 ([[gene|mgsA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA

BKK22480 ([[gene|mgsA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA

Biological materials

Expression vector

pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jörg Stülke]'s lab

pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Boris Görke], University of Vienna, Austria

[SW|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]

References

10368300, 21992469, 20308541, 22074179, 23894131

10368300, 21992469, 20308541, 22074179, 23894131, 28807600, 29514981

proteinLength

137

geneLength

414

Gene

Coordinates

2,358,911 2,359,324

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

MGS-like domain (aa 1-137) (according to UniProt)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jörg Stülke]'s lab

pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Boris Görke], University of Vienna, Austria

[SW|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]