Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-28 06:51:35
locus
BSU22480
BSU_22480
outlinks
bsu
BSU22480
BSU_22480
aminos
MKIALIAHDKKKQDMVQFTTAYRDILKNHDLYATGTTGLKIHEATGLQIERFQSGPLGGDQQIGALIAANALDLVIFLRDPLTAQPHEPDVSALIRLCDVYSIPLATNMGTAEILVRTLDEGVFEFRDLLRGEEPNV
[SW|Categories] containing this gene/protein
[SW|carbon core metabolism]
Gene
Coordinates on the chromosome (coding sequence)
2,358,911 -> 2,359,324
The protein
Catalyzed reaction/ biological activity
Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
dihydroxyacetone phosphate --> methylglyoxal + phosphate (according to UniProt)
The protein
Protein family
RNase Z family (according to Swiss-Prot)
methylglyoxal synthase family (single member, according to UniProt)
The protein
Effectors of protein activity
non-phosphorylated [[protein|Crh]] interacts with [[protein|MgsA]] to inhibit its activity [Pubmed|21992469]
non-phosphorylated [[protein|Crh]] interacts with [[protein|MgsA]] to inhibit its activity [Pubmed|29514981,21992469]
The protein
Structure
[PDB|1B93] (from ''Escherichia coli'', 50% identity, 73% similarity) [Pubmed|10368300]
[PDB|6F2C] [pubmed|29514981]
The protein
[SW|Interactions]
[[protein|Crh]]-[[protein|MgsA]] (only non-phosphorylated [[protein|Crh]] interacts with [[protein|MgsA]]) [Pubmed|21992469]
Expression and Regulation
Operon
''[[protein|ypjD]]-[[protein|dapB]]-[[protein|mgsA]]-[SW|bshB1]-[[protein|bshA]]-[[protein|cca]]-[[protein|birA]]'' [Pubmed|20308541]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|23894131]
Expression and Regulation
Regulation
induced by diamide stess (thiol depletion) ([[protein|Spx]]) [Pubmed|23894131]
Expression and Regulation
Regulatory mechanism
[[protein|Spx]]: transcription activation [Pubmed|23894131]
Biological materials
Mutant
GP67 (''mgsA''::''tet'') [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab
MGNA-A440 ([[gene|mgsA]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]
GP67 ([[gene|mgsA]]::''tet'') [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab
BKE22480 ([[gene|mgsA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
BKK22480 ([[gene|mgsA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
Biological materials
Expression vector
pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jörg Stülke]'s lab
pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Boris Görke], University of Vienna, Austria
[SW|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]
References
proteinLength
137
geneLength
414
Gene
Coordinates
2,358,911 2,359,324
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
MGS-like domain (aa 1-137) (according to UniProt)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jörg Stülke]'s lab
pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Boris Görke], University of Vienna, Austria
[SW|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]