SubtiBank SubtiBank
Version comparison:

Sat Apr 30 2016 19:53:12 GMT+0200 (CEST)2025-05-28 22:24:03

locus

BSU09420

BSU_09420

outlinks

bsu

BSU09420

BSU_09420

[SW|Categories] containing this gene/protein

[SW|cell wall degradation/ turnover], [SW|cell wall synthesis]

Gene

Coordinates on the chromosome (coding sequence)

1,018,998 -> 1,020,002

Gene

Phenotypes of a mutant

a ''[[protein|cwlO]] [[protein|lytE]]'' mutant is not viable [Pubmed|17581128,22139507]

growth defect at high temperature [Pubmed|21541672]

inactivation of ''[[protein|lytE]]'' strongly restores beta-lactam resistance in a ''[[protein|sigM]]'' mutant by delaying cell lysis [Pubmed|22211522]

a ''[[protein|lytE]]'' mutation is synthetically lethal with ''[[protein|ftsE]]'' and ''[[protein|ftsX]]'' mutation (due to a lack of autolysin activity) [Pubmed|23869552,23855774]

a ''[[protein|lytE]]'' mutation increases the cell separation defect of a ''[[protein|lytF]]'' mutant [Pubmed|23855774]

cells are thinner (reduced diameter) as compared to the wild type [Pubmed|23869552]

a ''[[gene|cwlO]] [[gene|lytE]]'' mutant is not viable [Pubmed|17581128,22139507]

a [[gene|ugtP]] [[gene|lytE]] double mutant has a severe [SW|cell shape] defect, thi can be suppressed by mutations resulting in reduced expression or activity of [[protein|PonA|PbpA]] [pubmed|33087775]

growth defect at high temperature [Pubmed|21541672]

inactivation of ''[[gene|lytE]]'' strongly restores beta-lactam resistance in a ''[[gene|sigM]]'' mutant by delaying cell lysis [Pubmed|22211522]

synthetically lethal with ''[[gene|ftsE]]'' and ''[[gene|ftsX]]'' mutation (due to a lack of autolysin activity) [Pubmed|23869552,23855774]

synthetically lethal with [[gene|sweC]] and [[gene|sweD]], this can be suppressed by point mutations in [[gene|ftsE]] or [[gene|ftsX]] [pubmed|31437162]

a ''[[gene|lytE]]'' mutation increases the cell separation defect of a ''[[gene|lytF]]'' mutant [Pubmed|23855774]

cells are thinner (reduced diameter) as compared to the wild type [Pubmed|23869552]

reduced colony size with accumulation of dead cells in the colonies [pubmed|29114240]

The protein

Catalyzed reaction/ biological activity

cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]

cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]

degradation of gamma-polyglutamic acid [pubmed|29458655]

The protein

Protein family

nlpC/p60 family (according to Swiss-Prot)

[SW|Peptidase C40 family] (according to UniProt)

The protein

Paralogous protein(s)

the C-terminal D,L-endopeptidase domains of [[protein|LytE]], [[protein|LytF]], [[protein|CwlS]], and [[protein|CwlO]] exhibit strong sequence similarity

[[this]]

The protein

[SW|Domains]

contains three N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]

C-terminal D,L-endopeptidase domain [Pubmed|22139507]

contains three N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]

C-terminal D,L-endopeptidase domain ([SW|NlpC/P60 domain]) [pubmed|29458655,22139507]

3 [SW|LysM domain]s (aa 26-69, aa 86-129, aa 149-192) (according to UniProt)

The protein

Effectors of protein activity

activity requires functional [[protein|MreB]] and [SW|MreBH] [Pubmed|23869552,16950129]

activity requires functional [[protein|MreB]] and [[protein|MreBH]] [Pubmed|23869552,16950129]

both enzymatic activities are inhibited by interaction with [[protein|IseA]] [pubmed|29458655]

The protein

[SW|Localization]

binds the cell wall [Pubmed|21261835]

localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) [Pubmed|22139507]

localization to lateral cell wall depends on the interaction with [SW|MreBH] [Pubmed|23869552]

binds the cell wall [Pubmed|21261835]

localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) [Pubmed|22139507]

localization to lateral cell wall depends on the interaction with [[protein|MreBH]] [Pubmed|23869552]

The protein

[SW|Interactions]

[SW|MreBH]-[[protein|LytE]] along the cylindrical part of the cell wall [Pubmed|16950129]

[[protein|FtsZ]]-[[protein|LytE]] at the division septum [Pubmed|16950129]

[[protein|PbpB]]-[[protein|LytE]] at the division septum [Pubmed|16950129]

Expression and Regulation

Operon

''lytE'' [Pubmed|9573210]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|9573210], [[protein|SigH]] [Pubmed|9573210], [[protein|SigI]] [Pubmed|21541672]

Expression and Regulation

Regulation

repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

induced at high temperature ([[protein|SigI]], [[protein|WalR]]) [Pubmed|24125693,21541672]

Expression and Regulation

Regulatory mechanism

[[protein|WalR]]: transcription activation [Pubmed|24125693,17581128]

[SW|Spo0A]: transcription repression [Pubmed|14651647]

Biological materials

Mutant

1A790 ( ''lytE''::''cat''), [Pubmed|9457885], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A790&Search=1A790 BGSC]

1A792 ( ''lytE''::''cat''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]

1A1024 ( ''lytE''::''spec''), [Pubmed|20400548], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1024&Search=1A1024 BGSC]

BKE09420 (''[[protein|lytE]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)

1A790 ( ''lytE''::''cat''), [Pubmed|9457885], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A790&Search=1A790 BGSC]

1A792 ( ''lytE''::''cat''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]

1A1024 ( ''lytE''::''spec''), [Pubmed|20400548], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1024&Search=1A1024 BGSC]

BKE09420 ([[gene|lytE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09420 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCTCCCCAAATG, downstream forward: _UP4_TAATTTTTAGAGAAAACCCG

BKK09420 ([[gene|lytE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCTCCCCAAATG, downstream forward: _UP4_TAATTTTTAGAGAAAACCCG

References

Original publications

21261835, 22211522, 16950129, 17581128, 14594841, 9457885, 9573210, 10322020, 24125693, 23199363, 20059685, 14651647, 23869552, 21541672, 22139507, 23855774

21261835, 22211522, 16950129, 17581128, 14594841, 9457885, 9573210, 10322020, 24125693, 23199363, 20059685, 14651647, 23869552, 21541672, 22139507, 23855774, 27118079, 25760608, 29114240, 29458655, 29914988, 29458657, 29465029, 31437162, 31808740, 33087775

proteinLength

334

geneLength

1005

Gene

Coordinates

1,018,998 1,020,002

The protein

Structure

[PDB|4XCM] (from Thermus thermophilus, 34% identity) [pubmed|25760608]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]