Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-24 03:07:55
locus
BSU38860
BSU_38860
outlinks
bsu
BSU38860
BSU_38860
aminos
MAILVTGGAGYIGSHTCVELLNSGYEIVVLDNLSNSSAEALNRVKEITGKDLTFYEADLLDREAVDSVFAENEIEAVIHFAGLKAVGESVAIPLKYYHNNLTGTFILCEAMEKYGVKKIVFSSSATVYGVPETSPITEDFPLGATNPYGQTKLMLEQILRDLHTADNEWSVALLRYFNPFGAHPSGRIGEDPNGIPNNLMPYVAQVAVGKLEQLSVFGNDYPTKDGTGVRDYIHVVDLAEGHVKALEKVLNSTGADAYNLGTGTGYSVLEMVKAFEKVSGKEVPYRFADRRPGDIATCFADPAKAKRELGWEAKRGLEEMCADSWRWQSSNVNGYKSAE
[SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources], [SW|biofilm formation]
Gene
Coordinates on the chromosome (coding sequence)
3,989,948 -> 3,990,967
Gene
Phenotypes of a mutant
smooth colonies on MsGG medium, no [SW|biofilm formation] in the absence of externally provided galactose [Pubmed|22893383,22113911]
the mutant is sensitive to exogenous galactose due to the accumulation of toxic UDP-galactose, this sensitivity can be suppressed by the inactivation of ''[[protein|galK]]'' (no formation of the toxic UDP-galactose) or of ''[[protein|sinR]]'' (constitutive expression of the ''[[protein|epsA]]-[[protein|epsB]]-[[protein|epsC]]-[[protein|epsD]]-[[protein|epsE]]-[[protein|epsF]]-[[protein|epsG]]-[[protein|epsH]]-[[protein|epsI]]-[[protein|epsJ]]-[[protein|epsK]]-[[protein|epsL]]-[[protein|epsM]]-[[protein|epsN]]-[[protein|epsO]]'' results in detoxification of UDP-galactose) [Pubmed|22893383]
smooth colonies on MsGG medium, no [SW|biofilm formation] in the absence of externally provided galactose [Pubmed|22893383,22113911]
the mutant is sensitive to exogenous galactose due to the accumulation of toxic UDP-galactose, this sensitivity can be suppressed by the inactivation of ''[[gene|galK]]'' (no formation of the toxic UDP-galactose) or of ''[[gene|sinR]]'' (constitutive expression of the ''[[gene|epsA]]-[[gene|epsB]]-[[gene|epsC]]-[[gene|epsD]]-[[gene|epsE]]-[[gene|epsF]]-[[gene|epsG]]-[[gene|epsH]]-[[gene|epsI]]-[[gene|epsJ]]-[[gene|epsK]]-[[gene|epsL]]-[[gene|epsM]]-[[gene|epsN]]-[[gene|epsO]]'' results in detoxification of UDP-galactose) [Pubmed|22893383]
The protein
Catalyzed reaction/ biological activity
UDP-glucose = UDP-galactose (according to Swiss-Prot)
UDP-α-D-glucose --> UDP-α-D-galactose (according to UniProt)
The protein
Protein family
sugar epimerase family (according to Swiss-Prot)
[SW|NAD(P)-dependent epimerase/dehydratase family] (according to UniProt)
Expression and Regulation
Operon
''galE'' [Pubmed|10746760]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|9555917]
Expression and Regulation
Regulation
strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]
proteinLength
339
geneLength
1020
Gene
Coordinates
3,989,948 3,990,967
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B736 (galE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1735 NBRP B. subtilis, Japan]
BKE38860 ([[gene|galE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTAAACCCTCCTAA, downstream forward: _UP4_TAAGAATGGAGGCCTTCTCA
BKK38860 ([[gene|galE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTAAACCCTCCTAA, downstream forward: _UP4_TAAGAATGGAGGCCTTCTCA