SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-12 21:17:24

locus

BSU37710

BSU_37710

outlinks

bsu

BSU37710

BSU_37710

aminos

MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHAALIEKYSVAVIKDKDYFKSLADFEHPDSIYWAHEDHNKPEEEVVEQIVKVAEMFGADAITTNNELFIAPMAKACERLGLRGAGVQAAENARDKNKMRDAFNKAGVKSIKNKRVTTLEDFRAALEEIGTPLILKPTYLASSIGVTLITDTETAEDEFNRVNDYLKSINVPKAVTFEAPFIAEEFLQGEYGDWYQTEGYSDYISIEGIMADGEYFPIAIHDKTPQIGFTETSHITPSILDEEAKKKIVEAAKKANEGLGLQNCATHTEIKLMKNREPGLIESAARFAGWNMIPNIKKVFGLDMAQLLLDVLCFGKDADLPDGLLDQEPYYVADCHLYPQHFKQNGQIPETAEDLVIEAIDIPDGLLKGDTEIVSFSAAAPGTSVDLTLFEAFNSIAAFELKGSNSQDVAESIRQIQQHAKLTAKYVLPV

[SW|Categories] containing this gene/protein

[SW|miscellaneous metabolic pathways], [SW|biosynthesis of antibacterial compounds]

Gene

Coordinates on the chromosome (coding sequence)

3,870,668 -> 3,872,086

The protein

Catalyzed reaction/ biological activity

ligation of anticapsin to L-Ala to form bacilysin, ultimate step in bacilysin biosynthesis [Pubmed|23317005]

ligation of anticapsin to L-Ala to form bacilysin, ultimate step in bacilysin biosynthesis [Pubmed|23317005]

ATP + L-alanine + L-anticapsin --> ADP + bacilysin + H+ + phosphate (according to UniProt)

Expression and Regulation

Operon

''[[protein|bacA]]-[[protein|bacB]]-[[protein|bacC]]-[[protein|bacD]]-[[protein|bacE]]-[[protein|bacF]]'' [Pubmed|12372825]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|19801406]

Expression and Regulation

Regulatory mechanism

[[protein|CodY]]: transcription repression [Pubmed|12372825,21709425]

[[protein|AbrB]]: transcription repression [Pubmed|12697329,21709425]

[[protein|ScoC]]: transcription repression [Pubmed|19801406]

proteinLength

472

geneLength

1419

Gene

Coordinates

3,870,668 3,872,086

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|ATP-grasp doamin] (aa 142-355) (according to UniProt)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B241 (ywfE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1240 NBRP B. subtilis, Japan]

BKE37710 ([[gene|bacD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCCATATGTAAAACACTCC, downstream forward: _UP4_ACGGCAAAGTATGTGCTGCC

BKK37710 ([[gene|bacD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCCATATGTAAAACACTCC, downstream forward: _UP4_ACGGCAAAGTATGTGCTGCC