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Tue Dec 13 2016 11:16:30 GMT+0100 (CET)2025-05-12 05:39:06

description

DNA integrity scanning protein, diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests DNA replication during B. subtilis spore outgrowth until the germinating spores genome is free of damage

DNA integrity scanning protein, diadenylate cyclase, delays [SW|sporulation] in the case of chromosome damage, the DisA-dependent checkpoint arrests [SW|DNA replication] during B. subtilis spore outgrowth until the germinating spores genome is free of damage

locus

BSU00880

BSU_00880

function

control of sporulation initiation

control of [SW|sporulation] initiation

outlinks

bsu

BSU00880

BSU_00880

aminos

MEKEKKGAKHELDLSSILQFVAPGTPLRAGMENVLRANTGGLIVVGYNDKVKEVVDGGFHINTAFSPAHLYELAKMDGAIILSDSGQKILYANTQLMPDATISSSETGMRHRTAERVAKQTGCLVIAISERRNVITLYQENMKYTLKDIGFILTKANQAIQTLEKYKTILDKTINALNALEFEELVTFSDVLSVMHRYEMVLRIKNEINMYIKELGTEGHLIKLQVIELITDMEEEAALFIKDYVKEKIKDPFVLLKELQDMSSYDLLDDSIVYKLLGYPASTNLDDYVLPRGYRLLNKIPRLPMPIVENVVEAFGVLPRIIEASAEELDEVEGIGEVRAQKIKKGLKRLQEKHYLDRQL

[SW|Categories] containing this gene/protein

[SW|cell division], [SW|metabolism of signalling nucleotides], [SW|sporulation/ other], [SW|general stress proteins (controlled by SigB)], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|heat shock proteins]

Gene

Coordinates on the chromosome (coding sequence)

107,476 -> 108,558

The protein

Catalyzed reaction/ biological activity

synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352,21566650]

synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352,21566650]

binds [[protein|RecA]] to inhibit its activity [pubmed|30916351]

2 ATP --> c-di-AMP + 2 diphosphate (according to UniProt)

The protein

Protein family

disA family (according to Swiss-Prot)

DisA family (single member, according to UniProt)

The protein

[SW|Domains]

contains a [SW|DAC domain] for the synthesis of c-di-AMP [Pubmed|18439896]

contains a [SW|DAC domain] for the synthesis of c-di-AMP [Pubmed|18439896]

[SW|DAC domain] (aa 11-149) (according to UniProt)

The protein

Effectors of protein activity

[[protein|RadA]] is an inhibitor of [[protein|DisA]] activity [Pubmed|23760274]

[[protein|RadA]] is an inhibitor of [[protein|DisA]] activity [Pubmed|23760274]

the presence of potassium results in enhanced activity [pubmed|28420751]

The protein

[SW|Localization]

see a [http://download.cell.com/mmcs/journals/0092-8674/PIIS0092867406005034.mmc12.avi video] showing the movement of DisA in the cell (in real time) [Pubmed|16713562]

forms discrete globular foci in germinating spres that colocalize with the nucleoid [Pubmed|24244006]

see a [http://download.cell.com/mmcs/journals/0092-8674/PIIS0092867406005034.mmc12.avi video] showing the movement of DisA in the cell (in real time) [Pubmed|16713562]

forms discrete globular foci in germinating spres that colocalize with the nucleoid [Pubmed|24244006]

forms rapidly moving focus that pauses at [[protein|RecA]]-mediated recombination intermediates upon induction of DNA damage during spore development [pubmed|30916351]

The protein

[SW|Interactions]

forms octamers

[[protein|RacA]]-[[protein|DisA]] [Pubmed|12493822]

[[protein|RadA]]-[[protein|DisA]] [Pubmed|23760274], this interaction result in the inhibition of [[protein|DisA]] activity [Pubmed|23760274]

Expression and Regulation

Operon

''[[protein|ctsR]]-[[protein|mcsA]]-[[protein|mcsB]]-[[protein|clpC]]-[[protein|radA]]-[[protein|disA]]'' [Pubmed|8793870]

''disA'' [Pubmed|17434969]

Expression and Regulation

[SW|Sigma factor]

''[[protein|ctsR]]'': [[protein|SigA]] [Pubmed|8793870], [[protein|SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]

''disA'': [[protein|SigM]] [Pubmed|17434969]

Expression and Regulation

Regulation

induced by stress ([[protein|SigB]]) [Pubmed|11544224], induced by heat ([[protein|CtsR]]) [Pubmed|9987115]

expressed during [SW|sporulation] in the mother cell [Pubmed|24244006]

expressed during germination and spore outgrowth [Pubmed|24244006]

Expression and Regulation

Regulatory mechanism

[[protein|CtsR]]: transcription repression [Pubmed|9987115]

Biological materials

Mutant

GP2142 (''[[protein|disA]]''::''tet''), available in [SW|Jörg Stülke]'s lab

BKG2 (''[[protein|radA]]-[[protein|disA]]::spc''), available in [SW|Jörg Stülke]'s lab

1A939 ( ''disA''::''tet''), [Pubmed|16713562], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A939&Search=1A939 BGSC]

MGNA-B932 ([[gene|disA]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1931 NBRP B. subtilis, Japan]

GP2142 (''Δ''''[[gene|disA]]''::''tet''), available in [SW|Jörg Stülke]'s lab

BKG2 (''[[gene|radA]]-[[gene|disA]]::spc''), available in [SW|Jörg Stülke]'s lab

1A939 ( ''disA''::''tet''), [Pubmed|16713562], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A939&Search=1A939 BGSC]

GP2222 ([[gene|cdaA]]::cat [[gene|cdaS]]::ermC [[gene|disA]]::''tet''), available in [SW|Jörg Stülke]'s lab, the mutant is only viable on minimal medium at low potassium concentration [pubmed|28420751]

BKE00880 ([[gene|disA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCTTTTTTCTCTTTTTCCA, downstream forward: _UP4_TGATTTCGGTTAAAACCTTA

BKK00880 ([[gene|disA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCTTTTTTCTCTTTTTCCA, downstream forward: _UP4_TGATTTCGGTTAAAACCTTA

GP2715 (''Δ''''[[gene|disA]]''::''spc''), available in [SW|Jörg Stülke]'s lab

GP2752 (''Δ''''[[gene|disA]]''::''cat''), available in [SW|Jörg Stülke]'s lab

GP2782 (''Δ''''[[gene|disA]]''::''kan''), available in [SW|Jörg Stülke]'s lab

Biological materials

Expression vector

IPTG inducible expression of His-''disA'' in ''E. coli'': pGP2563 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [SW|Jörg Stülke]'s lab

References

Reviews

22933559, 18714086, 25869574, 23812326

22933559, 18714086, 25869574, 23812326, 30224435, 32472931, 32603625

References

Original publications

24244006, 8793870, 9987115, 16713562, 23760274, 11544224, 17434969, 18439896, 16713555, 12493822, 21566650, 23192352, 23608499, 25616256, 26014055, 27150552

24244006, 8793870, 9987115, 16713562, 23760274, 11544224, 17434969, 18439896, 16713555, 12493822, 21566650, 23192352, 23608499, 25616256, 26014055, 27150552, 28420751, 28511132, 28961460, 29536659, 29588402, 30877841, 30916351

proteinLength

360

geneLength

1083

Gene

Coordinates

107,476 108,558

Gene

Phenotypes of a mutant

a [[gene|cdaA]] [[gene|disA]] double mutant or [[gene|cdaA]] [[gene|cdaS]] [[gene|disA]] triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) [pubmed|28420751]

reduced spore survival after infrared exposure [pubmed|28961460]

altered morphology on MSgg medium [pubmed|29588402]

plant root colonization defect [pubmed|29588402]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Cofactors]

Mn2+ [pubmed|26014055]

The protein

additional information

subject to Clp-dependent proteolysis upon glucose starvation [pubmed|17981983]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

IPTG inducible expression of His-''disA'' in ''E. coli'': pGP2563 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [SW|Jörg Stülke]'s lab