Tue Dec 13 2016 11:16:30 GMT+0100 (CET)2025-05-18 18:31:41
locus
BSU02390
BSU_02390
outlinks
bsu
BSU02390
BSU_02390
aminos
MNKLIEREKTVYYKEKPDPSSLGFGQYFTDYMFVMDYEEGIGWHHPRIAPYAPLTLDPSSSVFHYGQAVFEGLKAYRTDDGRVLLFRPDQNIKRLNRSCERMSMPPLDEELVLEALTQLVELEKDWVPKEKGTSLYIRPFVIATEPSLGVKASRSYTFMIVLSPVGSYYGDDQLKPVRIYVEDEYVRAVNGGVGFAKTAGNYAASLQAQRKANELGYDQVLWLDAIEKKYVEEVGSMNIFFVINGEAVTPALSGSILSGVTRASAIELIRSWGIPVREERISIDEVYAASARGELTEVFGTGTAAVVTPVGELNIHGKTVIVGDGQIGDLSKKLYETITDIQLGKVKGPFNWTVEV
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of amino acids]
Gene
Coordinates on the chromosome (coding sequence)
259,016 -> 260,086
The protein
Catalyzed reaction/ biological activity
l-leucine
2-oxoglutarate + L-leucine --> 4-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-isoleucine --> (S)-3-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-valine --> 3-methyl-2-oxobutanoate + L-glutamate (according to UniProt)
The protein
Protein family
class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
[SW|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|YwaA]]
[[this]]
Expression and Regulation
Operon
''ybgE'' [Pubmed|15060025]
Expression and Regulation
Regulation
repressed by casamino acids [Pubmed|12107147]
repressed during growth in the presence of branched chain amino acids ([[protein|CodY]]) [Pubmed|12618455]
Expression and Regulation
Regulatory mechanism
[[protein|CodY]]: transcription repression [Pubmed|12618455,21699902,18083814]
Biological materials
Mutant
a ''ybgE'' mutant and a ''[[protein|bcd]] [[protein|ybgE]] [[protein|ywaA]]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
MGNA-B939 (ybgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1938 NBRP B. subtilis, Japan]
a ''ybgE'' mutant and a ''[[gene|bcd]] [[gene|ybgE]] [[gene|ywaA]]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
BKE02390 ([[gene|ybgE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
BKK02390 ([[gene|ybgE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
Biological materials
lacZ fusion
pGP248 (in [[protein|pAC5]]), available in [SW|Jörg Stülke]'s lab
pGP248 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab
proteinLength
356
geneLength
1071
Gene
Coordinates
259,016 260,086
The protein
[SW|Cofactors]
PLP
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]