Mon May 16 2016 16:33:39 GMT+0200 (CEST)2025-05-28 14:22:22
locus
BSU06440
BSU_06440
outlinks
bsu
BSU06440
BSU_06440
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides], [SW|most abundant proteins]
Gene
Coordinates on the chromosome (coding sequence)
700,232 -> 701,527
The protein
Catalyzed reaction/ biological activity
N(6)-(1,2-dicarboxyethyl)AMP = fumarate AMP (according to Swiss-Prot)
N6-(1,2-dicarboxyethyl)-AMP --> AMP + fumarate (according to UniProt)
(2S)-2-[5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamido]succinate --> 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + fumarate (according to UniProt)
The protein
Protein family
Adenylosuccinate lyase subfamily (according to Swiss-Prot)
[SW|lyase 1 family] (according to UniProt)
The protein
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
Expression and Regulation
Operon
''[[protein|purE]]-[[protein|purK]]-[[protein|purB]]-[[protein|purC]]-[[protein|purS]]-[[protein|purQ]]-[[protein|purL]]-[[protein|purF]]-[[protein|purM]]-[[protein|purN]]-[[protein|purH]]-[[protein|purD]]'' [Pubmed|3036807]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|3036807]
Expression and Regulation
Regulation
repressed in the presence of purine nucleotides ([[protein|PurR]]) [Pubmed|7638212]
repressed in the presence of adenine or adenosine([[protein|PurR]]) [Pubmed|2536750]
repressed in the presence of guanine([[protein|G-box]]) [Pubmed|3036807]
Expression and Regulation
Regulatory mechanism
[PurR]]: transcription repression [Pubmed|7638212]
[[protein|PurR]]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]
[[protein|G-box]]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]
Expression and Regulation
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
References
proteinLength
431
geneLength
1296
Gene
Coordinates
700,232 701,527
Gene
Phenotypes of a mutant
poor growth [pubmed|28189581]
non-transformable [pubmed|28189581]
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE06440 ([[gene|purB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC
BKK06440 ([[gene|purB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC