SubtiBank SubtiBank
Version comparison:

Mon May 16 2016 16:33:39 GMT+0200 (CEST)2025-05-28 14:22:22

locus

BSU06440

BSU_06440

outlinks

bsu

BSU06440

BSU_06440

[SW|Categories] containing this gene/protein

[SW|biosynthesis/ acquisition of nucleotides], [SW|most abundant proteins]

Gene

Coordinates on the chromosome (coding sequence)

700,232 -> 701,527

The protein

Catalyzed reaction/ biological activity

N(6)-(1,2-dicarboxyethyl)AMP = fumarate AMP (according to Swiss-Prot)

N6-(1,2-dicarboxyethyl)-AMP --> AMP + fumarate (according to UniProt)

(2S)-2-[5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamido]succinate --> 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + fumarate (according to UniProt)

The protein

Protein family

Adenylosuccinate lyase subfamily (according to Swiss-Prot)

[SW|lyase 1 family] (according to UniProt)

The protein

Additional information

subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]

subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operon

''[[protein|purE]]-[[protein|purK]]-[[protein|purB]]-[[protein|purC]]-[[protein|purS]]-[[protein|purQ]]-[[protein|purL]]-[[protein|purF]]-[[protein|purM]]-[[protein|purN]]-[[protein|purH]]-[[protein|purD]]'' [Pubmed|3036807]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|3036807]

Expression and Regulation

Regulation

repressed in the presence of purine nucleotides ([[protein|PurR]]) [Pubmed|7638212]

repressed in the presence of adenine or adenosine([[protein|PurR]]) [Pubmed|2536750]

repressed in the presence of guanine([[protein|G-box]]) [Pubmed|3036807]

Expression and Regulation

Regulatory mechanism

[PurR]]: transcription repression [Pubmed|7638212]

[[protein|PurR]]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]

[[protein|G-box]]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]

Expression and Regulation

Additional information

subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

References

1722815, 3036807, 12923093, 15378759, 7638212

1722815, 3036807, 12923093, 15378759, 7638212, 28189581

proteinLength

431

geneLength

1296

Gene

Coordinates

700,232 701,527

Gene

Phenotypes of a mutant

poor growth [pubmed|28189581]

non-transformable [pubmed|28189581]

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE06440 ([[gene|purB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC

BKK06440 ([[gene|purB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC