SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-11 07:00:33

description

putative 2-5 RNA-ligase

similar to 2´-CPDase

locus

BSU29960

BSU_29960

function

RNA metabolism

nucleotide metabolism

product

putative 2-5 RNA-ligase

putative 2´-CPDase

ec

outlinks

bsu

BSU29960

BSU_29960

aminos

MPDIRPHYFIGVPIPEGIANPIYQAAKNEPILTFQKWVHPLDYHITLIFLGAADETQIKKLEGSLAEIASEIDPFSIKFGKIDVFGDRRKPRVLHLEPKKNKTLDRLREHTKQAVLQAGFQVEKRPYHPHMTLARKWTGEDGFPAHVPFESGEVSMMAERFSLFQIHLNQSPKYEEIFKFQLS

[SW|Categories] containing this gene/protein

[SW|poorly characterized/ putative enzymes]

Gene

Coordinates on the chromosome (coding sequence)

3,065,751 -> 3,066,302

The protein

Protein family

UPF0097 family (according to Swiss-Prot)

2H phosphoesterase superfamily (with [[protein|YjcG]], according to UniProt) [pubmed|31422053]

The protein

Paralogous protein(s)

[[protein|YjcG]]

[[this]]

References

17012785

17012785, 12798681, 31422053

proteinLength

183

geneLength

552

Gene

Coordinates

3,065,751 3,066,302

The protein

Catalyzed reaction/ biological activity

H2O + RNA(n)-2',3'-cyclic phosphate --> H+ + RNA(n)-2'- phosphate (according to UniProt)

The protein

Structure

[PDB|1IUH] (from Thermus thermophilus, 24% identity) [pubmed|12798681]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A431 (ytlP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/431 NBRP B. subtilis, Japan]

BKE29960 ([[gene|ytlP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCTGACACTCCCTT, downstream forward: _UP4_TAATTCATTGCGGAGTGGAG

BKK29960 ([[gene|ytlP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCTGACACTCCCTT, downstream forward: _UP4_TAATTCATTGCGGAGTGGAG