Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-26 09:25:42
description
oxidoreductase, modification of YydF
radical SAM epimerase, modification of [[protein|YydF]]
locus
BSU40170
BSU_40170
function
control of LiaR-LiaS activity
control of [[protein|LiaR]]-[[protein|LiaS]] activity
product
oxidoreductase
radical SAM epimerase
ec
outlinks
bsu
BSU40170
BSU_40170
aminos
MYNKTVSINLDSRCNASCDHCCFSSSPTSTTRMEKEYIRELVTEFAKNKTIQVISFTGGEVFLDYKFLKELMEIIKPYEKQITLISNGFWGLSKKKVQEYFHDMNSLNVIALTISYDEYHAPFVKSSSIKNILEHSRKYPDIDISLNMAVTKDKMSNHILEELGDSILGVKITKFPMISVGAAKTRIKQENIHKFYSLEDEDSLHCPGYDIVYHHDGEIYPCCSPAIFETKITLREEYNQSFERTVEKLNSNLLLFILRKEGFKWFLNILKENNKIEEFDIPYEFSSICGVCGSLFNSAEKINYFYPYMEKYYNENFKV
[SW|Categories] containing this gene/protein
[SW|transcription factors and their control]
Gene
Coordinates on the chromosome (coding sequence)
4,126,481 -> 4,127,440
The protein
[SW|Interactions]
[[protein|YydF]]-[[protein|YydG]] [Pubmed|23106164]
Expression and Regulation
Operon
''[[protein|yydF]]-[[protein|yydG]]-[[protein|yydH]]-[[protein|yydI]]-[[protein|yydJ]]'' [Pubmed|17921301]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|17921301]
Expression and Regulation
Regulation
repressed by glucose (5-fold) [Pubmed|12850135]
activated after transition to stationary phase([[protein|AbrB]]) [Pubmed|17921301]
Expression and Regulation
Regulatory mechanism
[[protein|AbrB]]: transcription repression [Pubmed|17921301]
Expression and Regulation
Additional information
the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] [PubMed|21815947]
References
Original publications
proteinLength
319
geneLength
960
Gene
Coordinates
4,126,481 4,127,440
The protein
Catalyzed reaction/ biological activity
epimerization of Val-36 and Ile-44 of [[protein|YydF]] to the D-form [pubmed|28644475]
The protein
Protein family
radical SAM enzymes [pubmed|28644475]
The protein
[SW|Cofactors]
two Fe-S clusters [pubmed|28644475]
SAM [pubmed|28644475]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B810 (yydG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1809 NBRP B. subtilis, Japan]
BKE40170 ([[gene|yydG]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATATCCCCCAACAA, downstream forward: _UP4_TATCCTTATATGGAAAAATA
BKK40170 ([[gene|yydG]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATATCCCCCAACAA, downstream forward: _UP4_TATCCTTATATGGAAAAATA