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Fri Mar 11 2016 17:32:29 GMT+0100 (CET)2025-05-27 15:41:15

title

spoIVCB

sigK

description

[SW|RNA polymerase ][SW|sporulation]-specific [SW|sigma factor ](sigma-K) (5' part of the interrupted [[gene|sigK]] gene), with [[gene|sigKC]]

locus

BSU25760

BSU_25760

product

RNA polymerase sporulation-specific sigma factor
(sigma-K) (N-terminal half)

[SW|RNA polymerase ][SW|sporulation]-specific [SW|sigma factor](sigma-K) (5' part of the gene)

ec

names

spoIVCB

cisB

sigK

sigK

sigKN

spoIVCB

outlinks

bsu

BSU25760

BSU_25760

[SW|Categories] containing this gene/protein

[SW|transcription], [SW|sigma factors and their control], [SW|sporulation proteins], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

2,652,993 -> 2,653,463

The protein

Protein family

sigma-70 factor family (according to Swiss-Prot)

[SW|Sigma-70 factor family] (according to UniProt)

The protein

[SW|Interactions]

[SW|SpoIVFB]-pro[[protein|SigK]] [Pubmed|23995631,23585539,19805276]

[[protein|SigK]]-([[protein|RpoB]]-[[protein|RpoC]])

Expression and Regulation

Operon

''[[protein|sigK]]'' (composed of ''[SW|spoIVCB]-[SW|spoIIIC]'') [Pubmed|2536191]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigE]] [Pubmed|15699190,2841290], [[protein|SigK]] [Pubmed|2841290]

Expression and Regulation

Regulation

expressed during sporulation in the mother cell ([[protein|SigE]], [[protein|SigK]], [[protein|GerE]], [SW|SpoIIID]) [Pubmed|15699190,2841290,10075739,7966271]

Expression and Regulation

Regulatory mechanism

[[protein|GerE]]: transcription repression [Pubmed|10075739]

[SW|SpoIIID]: transcription activation [Pubmed|7966271]

[SW|ScoC]: transcription repression [Pubmed|25983726]

Expression and Regulation

Additional information

[[protein|SigK]] is activated by processing of membrane-bound pro-[[protein|SigK]] by the [[protein|SpoIVFB]] metalloprotease [PubMed|23585539,19805276]

'spoIVCB' and '[[protein|spoIIIC]]' form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.

References

Reviews

20833318, 20836086

20833318, 20836086, 31350897

References

Original Publications

14526016, 17720779, 1900494, 10438769, 2492118, 1691789, 2536191, 2163341, 10931284, 1577688, 9852018, 1942049, 7814326, 8550479, 11959848, 9501233, 10400595, 2115401, 7592342, 1518043, 8226681, 9078383, 2514119, 10611287, 14523132, 19805276, 10075739, 7966271, 17890309, 23585539, 23995631, 25983726

14526016, 17720779, 1900494, 10438769, 2492118, 1691789, 2536191, 2163341, 10931284, 1577688, 9852018, 1942049, 7814326, 8550479, 11959848, 9501233, 10400595, 2115401, 7592342, 1518043, 8226681, 9078383, 2514119, 10611287, 14523132, 19805276, 10075739, 7966271, 17890309, 23585539, 23995631, 25983726, 26953342, 29180425, 30403663, 32515031

proteinLength

156

geneLength

471

Gene

Coordinates

2,652,993 2,653,463

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE25760 ([[gene|sigK]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTCACCTCCACAAAAG, downstream forward: _UP4_AAAGGGGGGTGCATACACCC

BKK25760 ([[gene|sigK]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTCACCTCCACAAAAG, downstream forward: _UP4_AAAGGGGGGTGCATACACCC