2019-07-30 08:49:332025-01-21 10:23:08
Gene
Phenotypes of a mutant
a [[gene|recD2]] [[gene|recG]] double mutant is not viable [pubmed|28527403]
a [[gene|recD2]] [[gene|recG]] double mutant is not viable [pubmed|28527403]
a [[gene|recG]] [[gene|radA]] double mutant is non-transformable with chromosomal DNA [pubmed|31350886]
The protein
Catalyzed reaction/ biological activity
binds and unwinds Holliday junction DNA [Pubmed|24770420]
binds and unwinds Holliday junction DNA [Pubmed|24770420]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Biological materials
Mutant
1A897 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A897&Search=1A897 BGSC]
BKE15870 ([[gene|recG]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAATACCCTTAATGTTAG, downstream forward: _UP4_TGAGTATCAGAAGTTTTTGG
BKK15870 ([[gene|recG]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAATACCCTTAATGTTAG, downstream forward: _UP4_TGAGTATCAGAAGTTTTTGG
1A897 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A897&Search=1A897 BGSC]
BKE15870 ([[gene|recG]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAATACCCTTAATGTTAG, downstream forward: _UP4_TGAGTATCAGAAGTTTTTGG
BKK15870 ([[gene|recG]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15870 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CCCAATACCCTTAATGTTAG, downstream forward: _UP4_TGAGTATCAGAAGTTTTTGG
References
The protein
[SW|Domains]
[SW|Helicase ATP-binding domain] (aa 271-432) (according to UniProt)
[SW|Helicase C-terminal domain] (aa 451-611) (according to UniProt)
The protein
[SW|Localization]
associated with the [SW|replisome] [pubmed|17853894]