2017-05-24 08:36:572025-04-14 01:53:07
description
C-terminal part of the split gene spsM (together with yodU), spore envelope polysaccharide biosynthesis, in B. subtilis 168 the gene is disrupted by the SP-beta prophage
C-terminal part of the split gene spsM (together with [[gene|yodU]]), legionaminic acid synthesis, in B. subtilis 168 the gene is disrupted by the [[category|SP-beta prophage]]
locus
BSU21670
BSU_21670
function
spore envelope polysaccharide biosynthesis
legionaminic acid synthesis
product
unknown
UDP-NAcGlcA inverting 4,6-dehydratase (C-terminal part)
ec
outlinks
bsu
BSU21670
BSU_21670
The protein
Paralogous protein(s)
[[protein|EpsC]] (for SpsM)
[[this]]
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
Biological materials
Mutant
MGNA-A061 (ypqP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/61 NBRP B. subtilis, Japan]
MGNA-A061 (ypqP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/61 NBRP B. subtilis, Japan]
BKE21670 (Δ[[gene|ypqP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGATACAGCTTTATCTGTTT, downstream forward: _UP4_TAAAAACAAGCCTGTCTATC
BKK21670 (Δ[[gene|ypqP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGATACAGCTTTATCTGTTT, downstream forward: _UP4_TAAAAACAAGCCTGTCTATC
References
Gene
Phenotypes of a mutant
the [[gene|ypqP]] mutant of PY79 has a less hydrophobic spore surface [pubmed|32817102]
The protein
Catalyzed reaction/ biological activity
SpsM catalyzes the C-4/C-6 dehydration of the UDP-GlcNAc to produce UDP-4-keto-6-deoxy-GlcNAc, this is the first reaction of the legionaminic acid biosynthesis pathway [pubmed|32817102]
required for spore crust assembly, legionaminic acid synthesis [pubmed|32817102]
The protein
Structure
[PDB|6BWC] (from B. thuringiensis, 39% identity) [pubmed|29266550]