SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:33 GMT+0100 (CET)2025-05-30 04:41:03

locus

BSU18210

BSU_18210

outlinks

bsu

BSU18210

BSU_18210

aminos

MLMDYEKERTERAERIRKGGAEKYHQSNREKGKLFVRERLSLLFDDDIELEDAFFAECMSDGLPADGVVTAIGKIGGQTVCVMANDSTVKAGSWGAKTVEKIIRIQEIAEKLNCPLIYLVDSAGARITDQINVFPGRRGAGRIFYNQVKLSGRIPQICLLFGPSAAGGAYIPAFCDIVVMVDGNASMYLGSPRMAEMVIGEKVSLEEMGGARMHCSISGCGDILAETEEEAIQLVRAYLSYFPANFQEKAPIHEKRPPKHFETPLADVIPQNQNAPFDMHELIERVIDEDSFFEIKALFAPELLTGLARIHGQPVGIVANQPKVKGGVLFHDSADKAAKFITLCDAFHIPLLFLADIPGFMIGTKVEQAGIIRHGAKMISAMSEATVPKLSVIVRKAYGAGLYAMAGPAFEPDCCLALPTAQIAVMGPEAAVNAVYAKKIAELPEEERAAFISSKREEYKEDINIYRLASEMIIDAVIPANSLRDELAKRLKAYMTKEMTFTNRKHPVYPV

[SW|Categories] containing this gene/protein

[SW|utilization of amino acids], [SW|sporulation proteins]

Gene

Coordinates on the chromosome (coding sequence)

1,949,682 -> 1,951,217

The protein

Catalyzed reaction/ biological activity

3-methylbut-2-enoyl-CoA + Biotin-CO2 ---> 3-methyl-glutaconyl-CoA + biotin [Pubmed|19935659]

3-methylbut-2-enoyl-CoA + Biotin-CO2 -- 3-methyl-glutaconyl-CoA + biotin [Pubmed|19935659]

The protein

Paralogous protein(s)

[[protein|YqjD]]

[[this]]

The protein

[SW|Interactions]

[[protein|YngH]]-[[protein|YngE]] [Pubmed|19935659]

Expression and Regulation

Operon

''[[protein|yngJ]]-[[protein|yngI]]-[[protein|yngH]]-[SW|yngHB]-[[protein|yngG]]-[[protein|yngF]]-[[protein|yngE]]'' [Pubmed|12662922]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigE]] [Pubmed|15699190,12662922]

Expression and Regulation

Regulation

expressed during sporulation in the mother cell ([[protein|SigE]]) [Pubmed|15699190,12662922]

proteinLength

511

geneLength

1536

Gene

Coordinates

1,949,682 1,951,217

The protein

Protein family

AccD/PCCB family (with [[protein|AccD]] and [[protein|YqjD]], according to UniProt)

The protein

[SW|Domains]

CoA carboxyltransferase N-terminal domain (aa 2-254) (according to UniProt)

CoA carboxyltransferase C-terminal domain (aa 260-506) (according to UniProt)

The protein

Structure

[PDB|1VRG] (from Thermotoga maritima, 40% identity)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B081 (yngE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1080 NBRP B. subtilis, Japan]

BKE18210 ([[gene|yngE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAATCCATCAGCATTGCCT, downstream forward: _UP4_TAAAAGCAAGAACTTAGGAG

BKK18210 ([[gene|yngE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAATCCATCAGCATTGCCT, downstream forward: _UP4_TAAAAGCAAGAACTTAGGAG