2017-05-24 12:54:352025-06-03 16:15:18
description
branched-chain amino acid transporter, major transporter for isoleucine and valine, may also be involved in threonine transport
amino acid transporter, major transporter for isoleucine, valine and threonine, minor serine transporter
locus
BSU09460
BSU_09460
geneLength
1395
1398
function
biosynthesis/acquisition of branched-chain amino acids
uptake of branched-chain amino acids, threonine, and serine
product
branched-chain amino acid transporter
amino acid transporter
ec
outlinks
bsu
BSU09460
BSU_09460
Gene
Coordinates
1,023,350 → 1,024,747
1,023,350 1,024,747
Gene
Phenotypes of a mutant
no phenotype for the single mutant, the triple ''[[gene|bcaP]] [[gene|brnQ]] [[gene|braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]
resistant to growth inhibition by high concentrations of threonine [Pubmed|25645558]
no phenotype for the single mutant, the triple ''[[gene|bcaP]] [[gene|brnQ]] [[gene|braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]
resistant to growth inhibition by high concentrations of threonine [Pubmed|32743959,25645558]
resistant to 4-hydroxythreonine [pubmed|25777134]
The protein
Catalyzed reaction/ biological activity
high affinity uptake of isoleucine and valine [Pubmed|25645558]
high affinity uptake of isoleucine and valine [Pubmed|25645558]
uptake of threonine [pubmed|32743959]
uptake of serine [pubmed|32743959]
The protein
Paralogous protein(s)
[[protein|MtrA]]
[[this]]
Biological materials
Mutant
MGNA-B479 (yhdG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1478 NBRP B. subtilis, Japan]
BKE09460 (''[[gene|bcaP]]''::''erm'', available in the BGSC and in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs)
MGNA-B479 (yhdG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1478 NBRP B. subtilis, Japan]
BKE09460 (''[[gene|bcaP]]''::''erm'', available in the BGSC and in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs) [pubmed|28189581]
BKE09460 ([[gene|bcaP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA, downstream forward: _UP4_TAACCTTTTGATAAAGAGAG
BKK09460 ([[gene|bcaP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA, downstream forward: _UP4_TAACCTTTTGATAAAGAGAG
References
The protein
Protein family
[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
The protein
Structure
[PDB|5OQT] (from Geobacillus kaustophilus, 60% identity) [pubmed|29416041]
Biological materials
Expression vector
pGP2298 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab