SubtiBank SubtiBank
Version comparison:

2017-05-24 12:54:352025-06-03 16:15:18

description

branched-chain amino acid transporter, major transporter for isoleucine and valine, may also be involved in threonine transport

amino acid transporter, major transporter for isoleucine, valine and threonine, minor serine transporter

locus

BSU09460

BSU_09460

geneLength

1395

1398

function

biosynthesis/acquisition of branched-chain amino acids

uptake of branched-chain amino acids, threonine, and serine

product

branched-chain amino acid transporter

amino acid transporter

ec

outlinks

bsu

BSU09460

BSU_09460

Gene

Coordinates

1,023,350 → 1,024,747

1,023,350 1,024,747

Gene

Phenotypes of a mutant

no phenotype for the single mutant, the triple ''[[gene|bcaP]] [[gene|brnQ]] [[gene|braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]

resistant to growth inhibition by high concentrations of threonine [Pubmed|25645558]

no phenotype for the single mutant, the triple ''[[gene|bcaP]] [[gene|brnQ]] [[gene|braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]

resistant to growth inhibition by high concentrations of threonine [Pubmed|32743959,25645558]

resistant to 4-hydroxythreonine [pubmed|25777134]

The protein

Catalyzed reaction/ biological activity

high affinity uptake of isoleucine and valine [Pubmed|25645558]

high affinity uptake of isoleucine and valine [Pubmed|25645558]

uptake of threonine [pubmed|32743959]

uptake of serine [pubmed|32743959]

The protein

Paralogous protein(s)

[[protein|MtrA]]

[[this]]

Biological materials

Mutant

MGNA-B479 (yhdG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1478 NBRP B. subtilis, Japan]

BKE09460 (''[[gene|bcaP]]''::''erm'', available in the BGSC and in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs)

MGNA-B479 (yhdG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1478 NBRP B. subtilis, Japan]

BKE09460 (''[[gene|bcaP]]''::''erm'', available in the BGSC and in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs) [pubmed|28189581]

BKE09460 ([[gene|bcaP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA, downstream forward: _UP4_TAACCTTTTGATAAAGAGAG

BKK09460 ([[gene|bcaP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA, downstream forward: _UP4_TAACCTTTTGATAAAGAGAG

References

18083814, 12618455, 20935095, 18763711, 21097623, 21699902, 25645558, 25777134

18083814, 12618455, 20935095, 18763711, 21097623, 21699902, 25645558, 25777134, 29416041, 32743959

The protein

Protein family

[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

The protein

Structure

[PDB|5OQT] (from Geobacillus kaustophilus, 60% identity) [pubmed|29416041]

Biological materials

Expression vector

pGP2298 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab