Tue Dec 13 2016 11:16:33 GMT+0100 (CET)2025-05-12 22:33:04
locus
BSU16950
BSU_16950
ec
outlinks
bsu
BSU16950
BSU_16950
aminos
MTSPTRRRTAKRRRRKLNKRGKLLFGLLAVMVCITIWNALHRNSEENEPSQETAAVSNTDQKKEVKKKTAKKSEEQIKTVDRNQKISNYLKEIGFSGTAMIVRNGEIVTNKGFGYADRKHYIQNNPLTSFYVGSSQKALIATAILQLEEKGKLQTSDPVSTYLPHFPNGQTITLKNLLTHTSGINGHIEGNGAITPDDLIKDIELQGIKRQPGVWDYKDSNYSVLAYIIAEVSGEPYEQYIKNHIFKPAGMTHAGFYKTYEKEPYPAVGYKMEGSKTVTPYIPDLSQLYGAGDIYMSAIDMYKFDQALIDGKLYSQKSYEKMFTPGSSSTYGMGFYVAPGSYSNHGVMPGFNILNSFSKSGQTIVILFSNIQNNAKLGQVNNKIYQLLNQE
[SW|Categories] containing this gene/protein
[SW|cell wall synthesis], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|penicillin-binding proteins]
Gene
Coordinates on the chromosome (coding sequence)
1,765,859 -> 1,767,034
Gene
Phenotypes of a mutant
increased sensitivity to lysozyme [Pubmed|21856855]
inactivation of ''[[protein|pbpX]]'' strongly restores beta-lactam resistance in a ''[[protein|sigM]]'' mutant [Pubmed|22211522]
increased sensitivity to lysozyme [Pubmed|21856855]
inactivation of ''[[gene|pbpX]]'' strongly restores beta-lactam resistance in a ''[[gene|sigM]]'' mutant [Pubmed|22211522]
The protein
[SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]
localizes during sporulation to both asymmetric septa and the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
extracellular (signal peptide) [Pubmed|18957862]
during vegetative growth: septal, low flourescence at periphery [pubmed|14731276]
during sporulation: both asymmetric septa and the prespore [pubmed|15758244]
Expression and Regulation
Operon
''pbpX'' [Pubmed|14762009]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigM]] [Pubmed|18179421], [[protein|SigX]] [Pubmed|14762009,15758244], [[protein|SigV]] [Pubmed|21926231]
Expression and Regulation
Regulation
expression is reduced in a [[protein|SigV]] mutant [Pubmed|21926231]
References
proteinLength
391
geneLength
1176
Gene
Coordinates
1,765,859 1,767,034
The protein
Protein family
[SW|beta-lactamase family] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3TG9] (a PBP from B. halodurans, aa 90-375, 28% identity)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B074 (pbpX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1073 NBRP B. subtilis, Japan]
BKE16950 ([[gene|pbpX]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
BKK16950 ([[gene|pbpX]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
Biological materials
GFP fusion
3107 [[gene|trpC]]2 [[gene|pbpX]]::pSG5047 (cat Pxyl-gfpa-[[gene|pbpX]]1-426) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]