SubtiBank SubtiBank
Version comparison:

Wed Sep 23 2015 18:43:08 GMT+0200 (CEST)2025-05-26 11:28:16

locus

BSU24340

BSU_24340

outlinks

bsu

BSU24340

BSU_24340

[SW|Categories] containing this gene/protein

[SW|biosynthesis of lipids], [SW|essential genes]

The protein

Catalyzed reaction/ biological activity

ATP biotin-carboxyl-carrier protein CO2 = ADP phosphate carboxybiotin-carboxyl-carrier protein (according to Swiss-Prot)

ATP + hydrogencarbonate + N6-biotinyl-L-lysyl-[protein] --> ADP + H+ + N6-carboxybiotinyl-L-lysyl-[protein] + phosphate (according to UniProt)

acetyl-CoA + ATP + hydrogencarbonate --> ADP + H+ + malonyl-CoA + phosphate (according to UniProt)

The protein

Paralogous protein(s)

[[protein|YotE]]

[[this]]

The protein

[SW|Interactions]

([[protein|AccA]]-[[protein|AccD]])-[[protein|AccB]]-[[protein|AccC]] (acetyl-CoA carboxylase complex)

Expression and Regulation

Operon

''[[protein|accB]]-[[protein|accC]]-[[protein|yqhY]]'' [Pubmed|7592499]

Biological materials

Mutant

GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

References

Original Publications

7592499, 7915138

7592499, 7915138, 28579978, 28516784

proteinLength

450

geneLength

1353

Gene

Coordinates

2,530,354 2,531,706

The protein

[SW|Domains]

[SW|ATP-grasp domain] (aa 120-318) (according to UniProt)

Biotin carboxylation domain (aa 1-447) (according to UniProt)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP1025, N-terminal Strep-tag, based on [SW|pGP172], purification from ''E. coli'', available in [SW|Jörg Stülke]'s lab