Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-10 16:31:45
title
yvcL
whiA
description
WhiA family protein, involved in Z ring assembly, putative transcription factor
WhiA family protein, involved in Z ring assembly, required for normal chromosome segregation
locus
BSU34750
BSU_34750
function
control of Z ring asembly
control of Z ring asembly and chromosome segregation
product
cell division protein
[[category|Cell division]] protein
ec
names
yvcL
whiA
yvcL
outlinks
bsu
BSU34750
BSU_34750
aminos
MSFASETKKELTNLEVKDCCINAELSALIRMNGALSFTNRHLVLDVQTENAAIARRIYTLLKKQYDVSVELLVRKKMRLKKNNVYIVRFSENAKAILEDLKILGENFVFERSISKELVKKRCCKRSYMRGAFLAGGSVNNPETSSYHLEIFSLYKEHNDSLCDLLNEFQLNSKTLERKKGYITYLKEAEKITEFLNVIGAHNSLLRFEDVRIVRDMRNSVNRLVNCETANLNKTIGASLRQVENIKYIDERIGLEALPEKLREIAQLRIDYQEVTLKELGEMVASGKISKSGINHRLRKLDEIAEQLRTGQTVTLK
[SW|Categories] containing this gene/protein
[SW|cell division], [SW|transcription factors and their control]
Gene
Coordinates on the chromosome (coding sequence)
3,569,573 -> 3,570,523
Gene
Phenotypes of a mutant
a ''[[protein|yvcL]] [[protein|zapA]]'' double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) [Pubmed|24097947]
a ''[[protein|yvcL]] [[protein|ezrA]]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
a ''[[protein|yvcL]] [[protein|minC]]-[[protein|minD]]'' mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
a ''[[protein|yvcL]] [[protein|noc]]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
the defects of the ''[[protein|yvcL]] [[protein|zapA]]'' double mutant can be suppressed by inactivation of either ''[[protein|gtaB]]'', ''[[protein|pgcA]]'', or ''[[protein|ugtP]]'' [Pubmed|24097947]
a [[gene|whiA]] [[gene|zapA]] double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) [Pubmed|24097947]
a [[gene|whiA]] [[gene|ezrA]] double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
a [[gene|whiA]] [[gene|minC]]-[[gene|minD]] mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
a [[gene|whiA]] [[gene|noc]] double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
a [[gene|whiA ]][[gene|parB ]]double mutant is not viable, this can be suppressed by inactivation of [[gene|yneA ]][pubmed|29378890]
a [[gene|whiA ]][[gene|yabA]] double mutant is not viable [pubmed|29378890]
the defects of the ''[[gene|whiA]] [[gene|zapA]]'' double mutant can be suppressed by inactivation of either ''[[gene|gtaB]]'', ''[[gene|pgcA]]'', or ''[[gene|ugtP]]'' [Pubmed|24097947]
highly sensitive for DNA damaging agents [pubmed|29378890]
The protein
Protein family
WhiA family [Pubmed|10986251]
WhiA family (single member, according to UniProt) [Pubmed|10986251]
Expression and Regulation
Operon
''[[protein|yvcI]]-[[protein|yvcJ]]-[[protein|yvcK]]-[[protein|yvcL]]-[[protein|crh]]-[[protein|yvcN]]'' [Pubmed|9237995]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|16272399]
Expression and Regulation
Regulation
constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]
very weak stimuation of expression by citrate and succinate [Pubmed|16272399]
Biological materials
Mutant
GP231 (''amyE::P[[protein|rpsJ]]-lacZ, yvcL::cat'') available in [SW| Jörg Stülke]'s lab; plasmid pGP1468 was used to disrupt the gene.
MGNA-B639 (yvcL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1638 NBRP B. subtilis, Japan]
GP231 (''amyE::P[[gene|rpsJ]]-lacZ, [[gene|whiA]]::cat'') available in [SW| Jörg Stülke]'s lab; plasmid pGP1468 was used to disrupt the gene.
BKE34750 (Δ[[gene|whiA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCCACCTCATTTCA, downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
BKK34750 (Δ[[gene|whiA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCCACCTCATTTCA, downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
Biological materials
Expression vector
pGP1469 for expression in ''B. subtilis'' (in [SW|pGP382], with C-terminal STREP-TAG), available in [SW| Jörg Stülke]'s lab
pGP2429 (N-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP2430 (C-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
References
proteinLength
316
geneLength
951
Gene
Coordinates
3,569,573 3,570,523
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
pGP1469 for expression in ''B. subtilis'' (in [SW|pGP382], with C-terminal STREP-TAG), available in [SW| Jörg Stülke]'s lab
pGP2429 (N-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP2430 (C-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab