Mon Aug 31 2015 13:57:30 GMT+0200 (CEST)2025-05-11 17:59:06

locus

BSU38460

BSU_38460

outlinks

bsu

BSU38460

BSU_38460

syns

ipa-9r, tyrS1, tyrT

[SW|Categories] containing this gene/protein

[SW|translation]

Gene

Phenotypes of a mutant

sensitive against D-Tyr [Pubmed|25733610]

a ''[[protein|tyrS]] [[protein|tyrZ]]'' double mutant is not viable [Pubmed|25733610]

sensitive against D-Tyr [Pubmed|25733610]

a ''[[gene|tyrS]] [[gene|tyrZ]]'' double mutant is not viable [Pubmed|25733610]

The protein

Catalyzed reaction/ biological activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)

[[protein|TyrZ]] is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than [[protein|TyrS]] [Pubmed|25733610]

ATP + L-tyrosine + tRNA^Tyr --> AMP + diphosphate + H⁺ + L-tyrosyl-tRNA^Tyr (according to UniProt)

[[protein|TyrZ]] is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than [[protein|TyrS]] [Pubmed|25733610]

The protein

Protein family

TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

[SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)

[SW|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)

Expression and Regulation

Operon

''[[protein|dtrR]]-[[protein|tyrZ]]'' [Pubmed|25733610]

Expression and Regulation

[SW|Sigma factor]

''[[protein|tyrZ]]'' [Pubmed|1735721]

Expression and Regulation

Regulation

induced by tyrosine limitation ([[protein|T-box]]) [Pubmed|25733610,19258532,1735721]

Expression and Regulation

Regulatory mechanism

[[protein|T-box]]: [SW|RNA switch], transcriptional antitermination [Pubmed|25733610,19258532,1735721]

[[protein|DtrR]]: transcriptional repression [Pubmed|25733610]

Biological materials

Mutant

QB53532 (aphA3), available in [SW|Jörg Stülke]'s lab

BKE38460 ([[gene|tyrZ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC, downstream forward: _UP4_TAATCTGATATTCCGAAACG

BKK38460 ([[gene|tyrZ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC, downstream forward: _UP4_TAATCTGATATTCCGAAACG

proteinLength

413

geneLength

1242

Gene

Coordinates

3,947,158 3,948,399

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|S4 RNA-binding domain] (aa 353-413) (according to UniProt)

The protein

Structure

[PDB|6BQY] (from Acinetobacter baumannii, 46% identity)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]