2017-8-11 16:0:32025-01-21 07:39:38
locus
BSU38460
BSU_38460
geneLength
1239
1242
outlinks
bsu
BSU38460
BSU_38460
Gene
Coordinates
3,947,158 → 3,948,399
3,947,158 3,948,399
The protein
Catalyzed reaction/ biological activity
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
[[protein|TyrZ]] is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than [[protein|TyrS]] [Pubmed|25733610]
ATP + L-tyrosine + tRNATyr --> AMP + diphosphate + H+ + L-tyrosyl-tRNATyr (according to UniProt)
[[protein|TyrZ]] is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than [[protein|TyrS]] [Pubmed|25733610]
The protein
Protein family
TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
[SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
[SW|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)
Biological materials
Mutant
QB53532 (aphA3), available in [SW|Jörg Stülke]'s lab
QB53532 (aphA3), available in [SW|Jörg Stülke]'s lab
BKE38460 ([[gene|tyrZ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC, downstream forward: _UP4_TAATCTGATATTCCGAAACG
BKK38460 ([[gene|tyrZ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC, downstream forward: _UP4_TAATCTGATATTCCGAAACG
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|S4 RNA-binding domain] (aa 353-413) (according to UniProt)
The protein
Structure
[PDB|6BQY] (from Acinetobacter baumannii, 46% identity)