2017-04-24 15:34:122025-05-22 14:08:07
locus
BSU22460
BSU_22460
geneLength
1131
1134
ec
outlinks
bsu
BSU22460
BSU_22460
Gene
Coordinates
2,357,078 → 2,358,211
2,357,078 2,358,211
The protein
Catalyzed reaction/ biological activity
UDP-GlcNAc L-malate = GlcNAc(α1→2)L-malate UDP [Pubmed|20308541]; also uses D-malate as a substrate, but with much lower affinity [Pubmed|20308541]
UDP-GlcNAc L-malate = GlcNAc(12)L-malate UDP [Pubmed|20308541]; also uses D-malate as a substrate, but with much lower affinity [Pubmed|20308541]
(S)-malate + UDP-N-acetyl-α-D-glucosamine --> (S)-malyl N-acetyl-α-D-glucosaminide + H+ + UDP (according to UniProt)
The protein
Protein family
NamA subfamily (according to Swiss-Prot)
[SW|Glycosyltransferase 1 family] (according to UniProt)
[SW|Glycosyltransferase 4 subfamily] (according to UniProt)
Biological materials
Mutant
MGNA-A410 (ypjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/410 NBRP B. subtilis, Japan]
''bshA::mls'' available in [SW|John_Helmann] lab
GP88 (''bshA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab
BKE22460 (''bshA''::''erm trpC2'') available from the Bacillus Genetic Stock Center
MGNA-A410 (ypjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/410 NBRP B. subtilis, Japan]
''bshA::mls'' available in [SW|John_Helmann] lab
GP88 (''bshA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab
BKE22460 ([[gene|bshA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCATTGTTCGCCCCCAAG, downstream forward: _UP4_GATTTAGCAGAACCGGAGTG
BKK22460 ([[gene|bshA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCATTGTTCGCCCCCAAG, downstream forward: _UP4_GATTTAGCAGAACCGGAGTG
References
The protein
Paralogous protein(s)
[[this]]
References
Reviews
References
Original Publications