2017-09-28 13:45:262025-05-26 22:03:43

locus

BSU23550

BSU_23550

geneLength

1317

1320

outlinks

bsu

BSU23550

BSU_23550

Gene

Coordinates

2,451,463 → 2,452,782

2,451,463 2,452,782

The protein

Catalyzed reaction/ biological activity

(S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate -→ pyruvate

(S)-malate + NAD⁺ --> CO₂ + NADH + pyruvate (according to UniProt)

H⁺ + oxaloacetate --> CO₂ + pyruvate (according to UniProt)

The protein

Protein family

malic enzymes family (according to Swiss-Prot)

[SW|malic enzymes family] (according to UniProt)

The protein

Paralogous protein(s)

[[protein|YtsJ]]

[[this]]

Biological materials

Mutant

MGNA-C412 (yqkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2410 NBRP B. subtilis, Japan]

GP1136 (cat) available in [SW|Stülke] lab

BKE23550 (Δ[[gene|mleA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC

BKK23550 (Δ[[gene|mleA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC

MGNA-C412 (yqkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2410 NBRP B. subtilis, Japan]

GP1136 (cat) available in [SW|Jörg Stülke]'s lab

BKE23550 ([[gene|mleA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC

BKK23550 ([[gene|mleA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC

The protein

[SW|Domains]

[SW|ACT domain] (aa 9-84) (according to UniProt)

The protein

Structure

[PDB|2A9F] (from Streptococcus pneumoniae, 42% identity from aa 90 - 435)