SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-26 22:03:43

locus

BSU23550

BSU_23550

outlinks

bsu

BSU23550

BSU_23550

aminos

MIAKHMIRTLMIETPSVPGNLGRVATAIGLLGGDIGEVETVKVGPNYTMRNITVQVENEEQLQEVIAAVQALGEGIRLHTVSDEVLSAHEGGKIQMKSKMPIRSLAELGRVYTPGVADVCRLIEKEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAGMPVMEGKAALFDQLAGISGIPILLDTSDPEEIIKTVKHISPGFSGILLEDIGSPHCFEIEDRLKEELNIPVMHDDQHGTAVVTLAAAISACRSAGVDLKEAKVGQIGLGAAGVAICRMFMAYGVNAVYGTDKSESAMNRLEQYGGQAVSSIEELMETCDIVIATTGVPGLIKPAFVRSGQVILALSNPKPEIEPEAALQAGAAYAADGRSVNNVLGFPGIFRGALNAKSTEINHDMLVAAAEAIAACTKQGDVVPQPLDSKVHHAVAAAVEHAALTAVK

[SW|Categories] containing this gene/protein

[SW|utilization of specific carbon sources]

Gene

Coordinates on the chromosome (coding sequence)

2,451,463 -> 2,452,782

The protein

Catalyzed reaction/ biological activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate

(S)-malate + NAD+ --> CO2 + NADH + pyruvate (according to UniProt)

H+ + oxaloacetate --> CO2 + pyruvate (according to UniProt)

The protein

Protein family

malic enzymes family (according to Swiss-Prot)

[SW|malic enzymes family] (according to UniProt)

The protein

Paralogous protein(s)

[[protein|YtsJ]]

[[this]]

Expression and Regulation

Operon

''[[protein|ansA]]-[[protein|ansB]]-[[protein|mleN]]-[[protein|mleA]]'' [Pubmed|22383849]

''[[protein|mleN]]-[[protein|mleA]]'' [Pubmed|22383849]

Expression and Regulation

[SW|Sigma factor]

''[[protein|ansA]]'': [[protein|SigA]] [Pubmed|1711029]

''[[protein|mleN]]'': [[protein|SigA]] [Pubmed|22900538]

Expression and Regulation

Regulation

''[[protein|ansA]]'': expressed in the presence of asparagine ([[protein|AnsR]]) [Pubmed|11914346]

''[[protein|mleN]]'': repressed by glucose ([[protein|CcpA]]) [Pubmed|22900538]

Expression and Regulation

Regulatory mechanism

[[protein|AnsR]]: transcription repression [Pubmed|11914346]

[[protein|CcpA]]: transcription repression [Pubmed|22900538]

Biological materials

Mutant

GP1136 (cat) available in [SW|Stülke] lab

MGNA-C412 (yqkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2410 NBRP B. subtilis, Japan]

GP1136 (cat) available in [SW|Jörg Stülke]'s lab

BKE23550 ([[gene|mleA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC

BKK23550 ([[gene|mleA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC

proteinLength

439

geneLength

1320

Gene

Coordinates

2,451,463 2,452,782

The protein

[SW|Domains]

[SW|ACT domain] (aa 9-84) (according to UniProt)

The protein

Structure

[PDB|2A9F] (from Streptococcus pneumoniae, 42% identity from aa 90 - 435)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]