low affinity potassium transporter KtrC-KtrD, peripheric membrane component

glutamate-controlled potassium channel [[protein|KtrC]]-[[protein|KtrD]], peripheric membrane component

BSU14510

BSU_14510

low affinity potassium transporter KtrC-KtrD,
peripheric membrane component (proton symport)

glutamate-controlled potassium channel [[protein|KtrC]]-[[protein|KtrD]], peripheric membrane component

BSU14510

BSU_14510

[SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]

1,520,531 -> 1,521,196

[[protein|KtrA]]

[[this]]

contains a [SW|RCK_N domain] at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK N-terminal domain* UniProt])

contains a c-di-AMP-binding [SW|RCK_C domain] at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK C-terminal domain* UniProt]) [Pubmed|23671116]

contains a [SW|RCK_N domain] at the N-terminus (aa 4-126)(according to [http://www.uniprot.org/uniprot/?query=RCK+N-terminal+domain&sort=score UniProt])

contains a c-di-AMP-binding [SW|RCK_C domain] at the C-terminus (aa 135-219) (according to [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score UniProt]) [Pubmed|23671116]

the protein binds c-di-AMP [Pubmed|23671116]

binds ADP and ATP [pubmed|30753894]

the protein binds c-di-AMP, K_D = 30 nM, this results in inhibition of potassium uptake [Pubmed|30753894]

the affinity of [[protein|KtrC]]-[[protein|KtrD]] for potassium is strongly increased in the presence of glutamate [pubmed|32253343]

[PDB|4J7C] (the [[protein|KtrA]]-[[protein|KtrB]] complex, 56% identity) [Pubmed|23598340]

[PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 57% identity) [Pubmed|25957408]

[PDB|4J7C] (the [[protein|KtrA]]-[[protein|KtrB]] complex, 56% identity) [Pubmed|23598340]

[PDB|6I8V] ([[protein|KtrC]] in complex with ATP) [Pubmed|30753894]

[PDB|4XTT] (the ''S. aureus'' [[protein|KtrA]] [SW|RCK_C domain] in complex with c-di-AMP, 57% identity) [Pubmed|25957408]

[[protein|KtrC]]-[[protein|KtrD]] [Pubmed|12562800]

''ktrC'' [Pubmed|12562800]

constitutively expressed [Pubmed|12562800]

MGNA-A904 (ykqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/904 NBRP B. subtilis, Japan]

GHB6 (''ktrC''::''spec''), [Pubmed|12562800] (available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s) labs, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A955&Search=1A955 BGSC] as 1A955

GP2264 (''[[protein|ktrC]]''::''ermC''), available in [SW|Jörg Stülke]'s lab

GP2079 (''[[protein|ktrC]]''::''tet''), available in [SW|Jörg Stülke]'s lab

GP2083 (D(''[[protein|ktrA]]-[[protein|ktrB]]'')::''aphA3'' D''[[protein|ktrC]]''::''tet''), available in [SW|Jörg Stülke]'s lab

MGNA-A904 (ykqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/904 NBRP B. subtilis, Japan]

GHB6 ([[gene|ktrC]]::''spec''), [Pubmed|12562800] (available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s) labs, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A955&Search=1A955 BGSC] as 1A955

GP2264 (''Δ''''[[gene|ktrC]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

GP2048 (''Δ''''[[gene|ktrC]]''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

GP2079 (''Δ''''[[gene|ktrC]]''::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

GP2771 (''Δ''''[[gene|ktrC]]''::''spec''), available in [SW|Jörg Stülke]'s lab

GP2083 ([[gene|ktrA]]-[[gene|ktrB]]::''aphA3'' [[gene|ktrC]]::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

BKE14510 ([[gene|ktrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC

BKK14510 ([[gene|ktrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC

pGP2907 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP2994: expression of Strep-KtrC in B. subtilis suitable for [SW|SPINE] (based on [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP2995: expression of KtrC-Strep in B. subtilis suitable for [SW|SPINE] (based on [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

25869574

25869574, 27935846, 25838295, 31361596, 32472931, 32603625

12562800, 23671116, 23598340

12562800, 23671116, 23598340, 28420751, 28504641, 30753894, 31061098, 31506295, 32253343

221

666

1,520,531 1,521,196

KtrA potassium transport family (with [[protein|KtrA]], according to UniProt)

the [[protein|KtrC]]-[[protein|KtrD ]]channel has a low affinity for potassium, this is determined by [[protein|KtrD]] [pubmed|30753894]

the affinity of [[protein|KtrC]]-[[protein|KtrD]] for potassium is strongly increased in the presence of glutamate (from 0.278 mM to 0.17 mM) [pubmed|32253343]

[[this]]

[[this]]

pGP2907 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi Homepage]

[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]

[SW|João H Morais-Cabral], University of Porto, Portugal [https://www.i3s.up.pt/research-group?x=47 Homepage]

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]