2017-8-11 16:0:32025-05-27 00:28:19
description
recombination protein, modulates the SOS response and facilitates RecA-mediated recombinational repair and genetic recombination
recombination protein, modulates the SOS response and facilitates [[protein|RecA]]-mediated recombinational repair and genetic recombination
locus
BSU08520
BSU_08520
geneLength
792
795
function
DNA repair/ recombination
[SW|DNA repair/ recombination]
ec
outlinks
bsu
BSU08520
BSU_08520
Gene
Coordinates
925,633 → 926,427
925,633 926,427
Gene
Phenotypes of a mutant
reduced natural tranformation with plasmid or chromosomal DNA [Pubmed|23284295]
drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
reduced natural transformation with plasmid or chromosomal DNA [Pubmed|23284295]
drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
The protein
Catalyzed reaction/ biological activity
modulates the "length or packing" of a [[protein|RecA]] filament, thereby [[protein|RecX]] facilitates the initiation of recombination and increases recombination across species [Pubmed|23284295]
modulates the "length or packing" of a [[protein|RecA]] filament, thereby [[protein|RecX]] facilitates the initiation of recombination and increases recombination across species [Pubmed|23284295]
inhibits [[protein|RecA]]-catalyzed ATP hydrolysis [pubmed|28911099]
inhibits stable [[protein|RecA]] nucleation and polymerization on ssDNA [pubmed|28911099]
induces [[protein|RecA]] nucleoprotein filament depolymerization [pubmed|28911099]
promotes a net [[protein|RecA]] disassembly from viral ssDNAs not homologous to the host genome [pubmed|31876108]
The protein
Protein family
recX family (according to Swiss-Prot)
recX family (single member, according to UniProt)
Biological materials
Mutant
MGNA-C361 (yfhG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2359 NBRP B. subtilis, Japan]
GP3533 (Δ[[gene|recX]]::kan), available in [SW|Jörg Stülke]'s lab
MGNA-C361 (yfhG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2359 NBRP B. subtilis, Japan]
BKE08520 ([[gene|recX]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAATCTCCCTTCTCA, downstream forward: _UP4_TTACTGCAGGAAGAGGAGTA
BKK08520 ([[gene|recX]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08520 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATCTTAATCTCCCTTCTCA, downstream forward: _UP4_TTACTGCAGGAAGAGGAGTA
References
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3D5L] (from Lactobacillus reuterii, 36% identity)