Sat May 21 2016 12:50:52 GMT+0200 (CEST)2025-05-22 12:57:16
locus
BSU31970
BSU_31970
outlinks
bsu
BSU31970
BSU_31970
[SW|Categories] containing this gene/protein
[SW|acquisition of iron], [SW|iron metabolism]
Gene
Coordinates on the chromosome (coding sequence)
3,287,675 -> 3,288,613
The protein
Catalyzed reaction/ biological activity
Isochorismate
Isochorismate + H2O --> 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate (according to UniProt)
The protein
Protein family
isochorismatase family (according to Swiss-Prot)
[SW|Isochorismatase family] (according to UniProt)
Expression and Regulation
Operon
''[[protein|dhbA]]-[[protein|dhbC]]-[[protein|dhbE]]-[[protein|dhbB]]-[[protein|dhbF]]'' [Pubmed|8921902]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|8550523]
Expression and Regulation
Regulation
induced by iron starvation ([[protein|Fur]]) [Pubmed|12354229]
strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]
Expression and Regulation
Regulatory mechanism
[[protein|Fur]]: transcription repression [Pubmed|12354229]
[[protein|AbrB]]: transcription repression [Pubmed|20817675]
Expression and Regulation
Additional information
the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] [PubMed|21815947]
the ''[[protein|dhbA]]-[[protein|dhbC]]-[[protein|dhbE]]-[[protein|dhbB]]-[[protein|dhbF]]'' operon is strongly unregulated in a ''[SW|kre]'' mutant [Pubmed|26110430]
References
proteinLength
312
geneLength
939
Gene
Coordinates
3,287,675 3,288,613
Gene
Phenotypes of a mutant
defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium [pubmed|31420537]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|Carrier domain] (aa 229-302) (according to UniProt)
The protein
Structure
[PDB|2FQ1] (from E. coli, 49% identity) [pubmed|16632253]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE31970 ([[gene|dhbB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAACACTTCCTCTCA, downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
BKK31970 ([[gene|dhbB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAACACTTCCTCTCA, downstream forward: _UP4_TTATAAAAGGAGGGTAAACA