Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-12 18:05:48
description
flottilin-like protein (in addition to FloT), resistence protein (against sublancin), accessory role in resistance to cefuroxime
flottilin-like protein (in addition to [[protein|FloT]]), resistence protein (against sublancin), accessory role in resistance to cefuroxime
locus
BSU25380
BSU_25380
function
resistance against sublancin
control of membrane fluidity
ec
outlinks
bsu
BSU25380
BSU_25380
aminos
MDPSTLMILAIVAVAIIVLAVFFTFVPVMLWISALAAGVKISIFTLVGMRLRRVIPNRVVNPLIKAHKAGLNVGTNQLESHYLAGGNVDRVVNALIAAQRANIELTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGIEVKAKARITVRANIERLVGGAGEETIVARVGEGIVSTIGSSDNHKKVLENPDMISQTVLGKGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMRARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSDEDRKS
[SW|Categories] containing this gene/protein
[SW|membrane dynamics], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|resistance against toxins/ antibiotics], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
2,617,449 -> 2,618,444
Gene
Phenotypes of a mutant
reduced [SW|protein secretion] [Pubmed|23651456]
a ''[[protein|floT]] [[protein|floA]]'' double mutant does not induce [[protein|KinC]]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
a ''[[protein|floT]] [[protein|floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
a ''[[protein|floT]] [[protein|floA]]'' double mutant has a [SW|sporulation] defect, due to the lack of [[protein|FtsH]] [Pubmed|22882210]
a ''[[protein|floA]]'' mutant produces increased amount of pulcherriminic acid [Pubmed|25909364]
a ''[[protein|floA]]'' mutant has reduced sensitivity to vancomycin [Pubmed|25909364]
reduced [SW|protein secretion] [Pubmed|23651456]
a ''[[gene|floT]] [[gene|floA]]'' double mutant does not induce [[protein|KinC]]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
a ''[[gene|floT]] [[gene|floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
a ''[[gene|floT]] [[gene|floA]]'' double mutant has a [SW|sporulation] defect, due to the lack of [[protein|FtsH]] [Pubmed|22882210]
a ''[[gene|floA]]'' mutant produces increased amount of pulcherriminic acid [Pubmed|25909364]
a ''[[gene|floA]]'' mutant has reduced sensitivity to vancomycin [Pubmed|25909364]
The protein
Protein family
UPF0365 family (according to Swiss-Prot)
UPF0365 family (single member, according to UniProt)
The protein
[SW|Interactions]
oligomerization depends on the glutamate-alanine repeats [Pubmed|25909364]
[[protein|FloA]]-[[protein|FtsH]] [Pubmed|22882210]
[[protein|FloT]]-[[protein|FloA]] [Pubmed|26297017,23651456]
[[protein|FloA]]-[[protein|PhoR]] [Pubmed|25909364]
Expression and Regulation
Operon
''[[protein|yqeZ]]-[[protein|floA]]-[[protein|yqfB]]'' [Pubmed|11866510]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigW]] [Pubmed|11866510]
Expression and Regulation
Regulation
expressed under stress conditions ([[protein|SigW]]) [Pubmed|11866510]
Biological materials
Mutant
JS119 (3610 markerless), available in [SW|Daniel Lopez]'s lab
DL1401 (168 ''ΔfloA''::mls), available in [SW|Daniel Lopez]'s lab
MGNA-C443 (yqfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2441 NBRP B. subtilis, Japan]
JS119 (3610 markerless), available in [SW|Daniel Lopez]'s lab
DL1401 (168 ''floA''::mls), available in [SW|Daniel Lopez]'s lab
BKE25380 ([[gene|floA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAACTTCTCCTCGTTT, downstream forward: _UP4_TAATCTCTCTAAGAAGGGAG
BKK25380 ([[gene|floA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAACTTCTCCTCGTTT, downstream forward: _UP4_TAATCTCTCTAAGAAGGGAG
References
Original publications
proteinLength
331
geneLength
996
Gene
Coordinates
2,617,449 2,618,444
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Expression and Regulation
additional information
the mRNA is cleaved by [[protein|Rnc|RNase III]] [PubMed|26883633]