SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-12 18:05:48

description

flottilin-like protein (in addition to FloT), resistence protein (against sublancin), accessory role in resistance to cefuroxime

flottilin-like protein (in addition to [[protein|FloT]]), resistence protein (against sublancin), accessory role in resistance to cefuroxime

locus

BSU25380

BSU_25380

function

resistance against sublancin

control of membrane fluidity

ec

outlinks

bsu

BSU25380

BSU_25380

aminos

MDPSTLMILAIVAVAIIVLAVFFTFVPVMLWISALAAGVKISIFTLVGMRLRRVIPNRVVNPLIKAHKAGLNVGTNQLESHYLAGGNVDRVVNALIAAQRANIELTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGIEVKAKARITVRANIERLVGGAGEETIVARVGEGIVSTIGSSDNHKKVLENPDMISQTVLGKGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMRARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSDEDRKS

[SW|Categories] containing this gene/protein

[SW|membrane dynamics], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|resistance against toxins/ antibiotics], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

2,617,449 -> 2,618,444

Gene

Phenotypes of a mutant

reduced [SW|protein secretion] [Pubmed|23651456]

a ''[[protein|floT]] [[protein|floA]]'' double mutant does not induce [[protein|KinC]]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]

a ''[[protein|floT]] [[protein|floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]

a ''[[protein|floT]] [[protein|floA]]'' double mutant has a [SW|sporulation] defect, due to the lack of [[protein|FtsH]] [Pubmed|22882210]

a ''[[protein|floA]]'' mutant produces increased amount of pulcherriminic acid [Pubmed|25909364]

a ''[[protein|floA]]'' mutant has reduced sensitivity to vancomycin [Pubmed|25909364]

reduced [SW|protein secretion] [Pubmed|23651456]

a ''[[gene|floT]] [[gene|floA]]'' double mutant does not induce [[protein|KinC]]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]

a ''[[gene|floT]] [[gene|floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]

a ''[[gene|floT]] [[gene|floA]]'' double mutant has a [SW|sporulation] defect, due to the lack of [[protein|FtsH]] [Pubmed|22882210]

a ''[[gene|floA]]'' mutant produces increased amount of pulcherriminic acid [Pubmed|25909364]

a ''[[gene|floA]]'' mutant has reduced sensitivity to vancomycin [Pubmed|25909364]

The protein

Protein family

UPF0365 family (according to Swiss-Prot)

UPF0365 family (single member, according to UniProt)

The protein

[SW|Interactions]

oligomerization depends on the glutamate-alanine repeats [Pubmed|25909364]

[[protein|FloA]]-[[protein|FtsH]] [Pubmed|22882210]

[[protein|FloT]]-[[protein|FloA]] [Pubmed|26297017,23651456]

[[protein|FloA]]-[[protein|PhoR]] [Pubmed|25909364]

Expression and Regulation

Operon

''[[protein|yqeZ]]-[[protein|floA]]-[[protein|yqfB]]'' [Pubmed|11866510]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigW]] [Pubmed|11866510]

Expression and Regulation

Regulation

expressed under stress conditions ([[protein|SigW]]) [Pubmed|11866510]

Biological materials

Mutant

JS119 (3610 markerless), available in [SW|Daniel Lopez]'s lab

DL1401 (168 ''ΔfloA''::mls), available in [SW|Daniel Lopez]'s lab

MGNA-C443 (yqfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2441 NBRP B. subtilis, Japan]

JS119 (3610 markerless), available in [SW|Daniel Lopez]'s lab

DL1401 (168 ''floA''::mls), available in [SW|Daniel Lopez]'s lab

BKE25380 ([[gene|floA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAACTTCTCCTCGTTT, downstream forward: _UP4_TAATCTCTCTAAGAAGGGAG

BKK25380 ([[gene|floA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAACTTCTCCTCGTTT, downstream forward: _UP4_TAATCTCTCTAAGAAGGGAG

References

Original publications

16629676, 11866510, 18763711, 20713508, 22753055, 22882210, 23651456, 22178969, 24222488, 25909364, 26297017, 27362352

16629676, 11866510, 18763711, 20713508, 22753055, 22882210, 23651456, 22178969, 24222488, 25909364, 26297017, 27362352, 26883633, 32662773

proteinLength

331

geneLength

996

Gene

Coordinates

2,617,449 2,618,444

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Expression and Regulation

additional information

the mRNA is cleaved by [[protein|Rnc|RNase III]] [PubMed|26883633]