2018-12-03 16:17:222025-04-06 20:08:41
locus
BSU32260
BSU_32260
geneLength
1299
1302
outlinks
bsu
BSU32260
BSU_32260
Gene
Coordinates
3,314,828 → 3,316,129
3,314,828 3,316,129
The protein
Catalyzed reaction/ biological activity
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H (according to Swiss-Prot)
L-aspartate 4-semialdehyde + NAD(P)H+H+ --> L-homoserine + NAD(P)+ [pubmed|6786715]
The protein
Protein family
homoserine dehydrogenase family (according to Swiss-Prot)
Homoserine dehydrogenase family (single member, according to UniProt)
The protein
Effectors of protein activity
subject to feedback inhibition [Pubmed|19258532]
The protein
[SW|Localization]
membrane associated [Pubmed|18763711]
Biological materials
Mutant
BKE32260 (Δ[[gene|hom]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
BKK32260 (Δ[[gene|hom]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
BKE32260 ([[gene|hom]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
BKK32260 ([[gene|hom]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
References
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|ACT domain] (aa 350-426) (according to UniProt)
The protein
[SW|Cofactors]
NADPH [pubmed|6786715]
Biological materials
Expression vector
pGP2297 (expression with SUMO protein and N-terminal His-tag from ''E. coli'', in [SW|pET-SUMOadapt]), available in [SW|Jörg Stülke]'s lab
pGP2300 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab