SubtiBank SubtiBank
Version comparison:

2018-12-03 16:17:222025-04-06 20:08:41

locus

BSU32260

BSU_32260

geneLength

1299

1302

outlinks

bsu

BSU32260

BSU_32260

Gene

Coordinates

3,314,828 → 3,316,129

3,314,828 3,316,129

The protein

Catalyzed reaction/ biological activity

L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H (according to Swiss-Prot)

L-aspartate 4-semialdehyde + NAD(P)H+H+ --> L-homoserine + NAD(P)+ [pubmed|6786715]

The protein

Protein family

homoserine dehydrogenase family (according to Swiss-Prot)

Homoserine dehydrogenase family (single member, according to UniProt)

The protein

Effectors of protein activity

subject to feedback inhibition [Pubmed|19258532]

The protein

[SW|Localization]

membrane associated [Pubmed|18763711]

Biological materials

Mutant

BKE32260 (Δ[[gene|hom]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG

BKK32260 (Δ[[gene|hom]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG

BKE32260 ([[gene|hom]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG

BKK32260 ([[gene|hom]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG

References

12107147, 18763711, 3139660, 19258532, 24163341, 25777134, 15378759, 25755103, 27260660

12107147, 18763711, 3139660, 19258532, 24163341, 25777134, 15378759, 25755103, 27260660, 6786715, 33046675

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|ACT domain] (aa 350-426) (according to UniProt)

The protein

[SW|Cofactors]

NADPH [pubmed|6786715]

Biological materials

Expression vector

pGP2297 (expression with SUMO protein and N-terminal His-tag from ''E. coli'', in [SW|pET-SUMOadapt]), available in [SW|Jörg Stülke]'s lab

pGP2300 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab