2017-12-15 17:34:152025-05-09 19:21:52

description

sigma factor of the RNA polymerase, Sigma-54, Sigma L

[SW|sigma factor ]of the [SW|RNA polymerase], Sigma-54, Sigma L

locus

BSU34200

BSU_34200

geneLength

1308

1311

product

RNA polymerase sigma-54 factor (sigma-L)

[SW|RNA polymerase] sigma-54 factor (sigma-L)

outlinks

bsu

BSU34200

BSU_34200

Gene

Coordinates

3,512,498 → 3,513,808

3,512,498 3,513,808

Gene

Phenotypes of a mutant

The mutant is cold-sensitive and unable to use arginine as a single carbon source. [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774 PubMed]

The mutant is cold-sensitive and unable to use arginine as a single carbon source [pubmed|16585774]

The protein

Protein family

sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family

sigma-54 factor family (single member, according to UniProt)

The protein

[SW|Domains]

DNA binding domain (H-T-H motif) (324–343)

pron box domain (413–421)

3 x Compositional bias domain (6–21),(32–53),(112–136)

DNA binding domain (HTH motif) (324343)

pron box domain (413421)

3 x Compositional bias domain (621),(3253),(112136)

The protein

Additional information

Transcription initiation by SigL-containing [SW|RNA polymerase] requires the activity of ATP-hydrolyzing transcription activators.

Transcription initiation by SigL-containing [SW|RNA polymerase] requires the activity of ATP-hydrolyzing transcription activators

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000078 PRODORIC2 database]

Biological materials

Mutant

GP146 (''sigL''::spc), available in [SW|Jörg Stülke]'s lab [Pubmed|17183217]

GP2302 (''sigL''::tet), available in [SW|Jörg Stülke]'s lab

1A914 (''sigL''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC]

BKE34200 (Δ[[gene|sigL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGCTCACTCCCCTTT, downstream forward: _UP4_TAAAATCCTCCCTAGACGGG

BKK34200 (Δ[[gene|sigL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGCTCACTCCCCTTT, downstream forward: _UP4_TAAAATCCTCCCTAGACGGG

GP146 (Δ''sigL''::spc), available in [SW|Jörg Stülke]'s lab [Pubmed|17183217]

GP2302 (Δ''sigL''::tet), available in [SW|Jörg Stülke]'s lab

1A914 (''sigL''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC]

BKE34200 (Δ[[gene|sigL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGCTCACTCCCCTTT, downstream forward: _UP4_TAAAATCCTCCCTAGACGGG

BKK34200 (Δ[[gene|sigL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGCTCACTCCCCTTT, downstream forward: _UP4_TAAAATCCTCCCTAGACGGG

Labs working on this gene/protein

[SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

The protein

Paralogous protein(s)

[[this]]

labs