2018-10-05 10:15:052025-05-16 10:18:30

description

tRNA^Met cytidine acetate ligase, required for proper decodign of the AUA elongator Met codon

tRNA^Met cytidine acetate ligase, required for proper decoding of the AUA elongator Met codon

locus

BSU15060

BSU_15060

geneLength

1245

1248

outlinks

bsu

BSU15060

BSU_15060

Gene

Coordinates

1,573,807 → 1,575,054

1,573,807 1,575,054

The protein

Catalyzed reaction/ biological activity

acetylation of the first position of the anticodon (position 34) of elongator tRNA^Met [pubmed|30150682]

acetate + ATP + cytidine³⁴ in elongator tRNA^Met --> AMP + diphosphate + N⁴-acetylcytidine³⁴ in elongator tRNA^Met (according to UniProt)

The protein

Protein family

UPF0348 family (according to Swiss-Prot)

TmcAL family (single member, according to UniProt)

Biological materials

Mutant

MGNA-B250 (ylbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1249 NBRP B. subtilis, Japan]

BKE15060 (Δ[[gene|tmcAL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAACCACCAATCCGACAG, downstream forward: _UP4_AATGTATAGAAAAAGCACCT

BKK15060 (Δ[[gene|tmcAL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAACCACCAATCCGACAG, downstream forward: _UP4_AATGTATAGAAAAAGCACCT

MGNA-B250 (ylbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1249 NBRP B. subtilis, Japan]

BKE15060 ([[gene|tmcAL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAACCACCAATCCGACAG, downstream forward: _UP4_AATGTATAGAAAAAGCACCT

BKK15060 ([[gene|tmcAL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAACCACCAATCCGACAG, downstream forward: _UP4_AATGTATAGAAAAAGCACCT