2020-07-10 08:57:002025-05-11 13:00:34
Biological materials
Mutant
MGNA-B441 (pgpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1440 NBRP B. subtilis, Japan]
BKE19650 ([[gene|pgpB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
BKK19650 ([[gene|pgpB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
GP3652 Δ[[gene|pgpB]]::cat, available in [SW|Jörg Stülke]'s lab
GP3652 Δ[[gene|pgpB]]::cat, available in [SW|Jörg Stülke]'s lab
MGNA-B441 (pgpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1440 NBRP B. subtilis, Japan]
BKE19650 ([[gene|pgpB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
BKK19650 ([[gene|pgpB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
Biological materials
Expression vector
pGP3502: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in pETSUMOadapt (available at Thermo Fisher), available in [SW|Jörg Stülke]'s lab
pGP3503: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
pGP3506 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP3502: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [https://www.thermofisher.com/order/catalog/product/K30001#/K30001 pETSUMOadapt], available in [SW|Jörg Stülke]'s lab
pGP3503: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
pGP3506 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab