title

yodM

pgpB

description

minor undecaprenyl pyrophosphate phosphatase, not active under standard conditions

phosphatidylglycerol phosphate (PGP) phosphatase , has minor undecaprenyl pyrophosphate phosphatase activity

function

unknown

phospholipid biosynthesis

product

minor undecaprenyl pyrophosphate phosphatase

phosphatidylglycerol phosphate (PGP) phosphatase

names

pgpB

yodM

Biological materials

Mutant

MGNA-B441 (pgpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1440 NBRP B. subtilis, Japan]

BKE19650 ([[gene|pgpB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC

BKK19650 ([[gene|pgpB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC

GP3652 Δ[[gene|pgpB]]::cat, available in [SW|Jörg Stülke]'s lab

MGNA-B441 (pgpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1440 NBRP B. subtilis, Japan]

BKE19650 ([[gene|pgpB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC

BKK19650 ([[gene|pgpB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC

The protein

Catalyzed reaction/ biological activity

1,2-diacyl-sn-glycero-3-phospho-(1ʼ-sn-glycero-3ʼ-phosphate) + H₂O --> 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate (according to UniProt)

Biological materials

Expression vector

pGP3502: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [https://www.thermofisher.com/order/catalog/product/K30001#/K30001 pETSUMOadapt], available in [SW|Jörg Stülke]'s lab

pGP3503: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP3506 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab