locus

BSU10300

BSU_10300

geneLength

1143

1146

product

extracellular alkaline serine protease (subtilisin E))

extracellular alkaline serine protease (subtilisin E)

outlinks

bsu

BSU10300

BSU_10300

Gene

Coordinates

1,104,423 → 1,105,568

1,104,423 1,105,568

The protein

Catalyzed reaction/ biological activity

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides(according to UniProt)

The protein

Protein family

peptidase S8 family (according to Swiss-Prot)

[SW|peptidase S8 family] (according to UniProt)

Biological materials

Mutant

KO7 (''ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr''), available as BGSC 1A1133

BKE10300 (Δ[[gene|aprE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT, downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG

BKK10300 (Δ[[gene|aprE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT, downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG

KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133

BKE10300 ([[gene|aprE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT, downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG

BKK10300 ([[gene|aprE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT, downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG

References

Original publications

19710937, 3082852, 2447063, 2447062, 19251843, 18414485, 6322190, 12884008, 15126467, 1898931, 1906467, 2504584, 18957862, 11101663, 12055299, 3131303, 1906467, 15598897, 11325965, 14688235, 21099137, 22745669, 23563549, 23563567, 24115457, 25315053, 12950930, 26728191

19710937, 3082852, 2447063, 2447062, 19251843, 18414485, 6322190, 12884008, 15126467, 1898931, 1906467, 2504584, 18957862, 11101663, 12055299, 3131303, 1906467, 15598897, 11325965, 14688235, 21099137, 22745669, 23563549, 23563567, 24115457, 25315053, 12950930, 26728191, 28800172, 29449835, 24279884

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

Inhibitor I9 domain (aa 38-109) (according to UniProt)

[SW|Peptidase S8 domain] (aa 111-380) (according to UniProt)

Expression and Regulation

additional information

expression is increased in a [[gene|hag]] mutant due to the presence of increased levels of [[protein|DegU]]-P [pubmed|28800172]

the mRNA is very stable (> 15 min) [pubmed|12884008]