SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:57:04 GMT+0200 (CEST)2025-05-10 04:57:58

locus

BSU35450

BSU_35450

ec

outlinks

bsu

BSU35450

BSU_35450

syns

[SW|Categories] containing this gene/protein

[SW|genetic competence], [SW|phosphoproteins]

The protein

Protein family

comF/gntX family (according to Swiss-Prot)

comF/gntX family (single member, according to UniProt)

Expression and Regulation

Operon

''[SW|comFA]-[SW|comFB]-[SW|comFC]-[[protein|yvyF]]-[[protein|flgM]]-[[protein|flgN]]-[[protein|flgK]]-[[protein|flgL]]'' [Pubmed|8412657]

Expression and Regulation

[SW|Sigma factor]

''[SW|comFA]'': [[protein|SigA]] [Pubmed|8412657]

Expression and Regulation

Regulatory mechanism

[[protein|ComK]]: transcription activation [Pubmed|8412657]

Biological materials

Expression vector

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

References

11814663, 8412657, 21278288, 22517742

11814663, 8412657, 21278288, 22517742, 28618091, 30254116

proteinLength

229

geneLength

690

Gene

Coordinates

3,641,125 3,641,814

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE35450 ([[gene|comFC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCGAATCACATAATAAAC, downstream forward: _UP4_TAAATGATTCTGTTTTTATG

BKK35450 ([[gene|comFC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCGAATCACATAATAAAC, downstream forward: _UP4_TAAATGATTCTGTTTTTATG

Biological materials

Expression vectors

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab