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yxeN [2018-03-05 09:27:27]
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yxeN [2018-03-05 09:27:27]

putative cysteine ABC transporter (permease), the yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ operon may be implicated in the utilization of a modified cysteine variant
locus
BSU39490
pI
9.48
mw
24.86 kDa
protein length
224 aa Sequence Blast
gene length
672 bp Sequence Blast
function
uptake of cysteine
product
putative cysteine ABC transporter (permease)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.3|Conversion of S-(2-succino)cysteine to cysteine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    4,058,791 → 4,059,465

    The protein

    Protein family

  • binding-protein-dependent transport system permease family (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

    • additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B710 (yxeN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1709 NBRP B. subtilis, Japan]
  • 1A950 ( ''yxeN''::''cat''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A950&Search=1A950 BGSC]
  • BKE39490 (Δ[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC, downstream forward: _UP4_TACCGGACTTAGAGGTGAAA
  • BKK39490 (Δ[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC, downstream forward: _UP4_TACCGGACTTAGAGGTGAAA

    • References

  • 10746760,10092453,16513748,18763711