SubtiBank SubtiBank
yxeN
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yxeN

putative S-(2-succino)cysteine ABC transporter (permease)
Locus
BSU_39490
Isoelectric point
9.48
Molecular weight
24.86 kDa
Protein length
224 aa Sequence Blast
Gene length
675 bp Sequence Blast
Function
uptake and utilization of S-(2-succino)cysteine
Product
putative S-(2-succino)cysteine ABC transporter (permease)
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
4,058,791 → 4,059,465

The protein

Protein family

Paralogous protein(s)

Domains

  • ABC transmembrane type-1 domain (aa 20-211) (according to UniProt)
  • Structure

  • 4YMS (amino acid transporter from Caldanaerobacter subterraneus, 39% identity) PubMed
  • Localization

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • Regulation

  • repressed in the presence of cysteine (CymR) PubMed
  • Additional information

  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B710 (yxeN::erm), available at the NBRP B. subtilis, Japan
  • 1A950 ( yxeN::cat), PubMed, available at BGSC
  • BKE39490 (ΔyxeN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC, downstream forward: _UP4_TACCGGACTTAGAGGTGAAA
  • BKK39490 (ΔyxeN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC, downstream forward: _UP4_TACCGGACTTAGAGGTGAAA
  • References

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