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yoaB [2019-06-19 11:57:36]
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yoaB [2019-06-19 11:57:36]

2-oxoglutarate permease (proton symporter)
locus
BSU18540
pI
9.41
mw
45.22 kDa
protein length
414 aa Sequence Blast
gene length
1245 bp Sequence Blast
function
uptake of alpha-ketoglutarate
product
2-oxoglutarate permease (proton symporter)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,021,223 → 2,022,467

    The protein

    Protein family

  • [SW|major facilitator superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|12B260DBC4E29151364251837FD227593D0BF391|CsbX]

    • [SW|Localization]

  • membrane [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|3769B79A180412C0FE98B3FC54BA49750BAFEDF6|yoaD]-[gene|182B8EFA41DAE695B008CBAA561F3730D11750B1|yoaC]-[gene|FF60633F2902E4CA58B34FF3DAFF85B0D4007622|yoaB]
    description
    [Pubmed|10094622]

    regulatory mechanism

  • [regulon|S-box|S-box]: termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

    • regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A833 (yoaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/833 NBRP B. subtilis, Japan]
  • BKE18540 (Δ[gene|FF60633F2902E4CA58B34FF3DAFF85B0D4007622|yoaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGTCTCCTCCTTAG, downstream forward: _UP4_TAATTAGAAAGCGCCCTGTT
  • BKK18540 (Δ[gene|FF60633F2902E4CA58B34FF3DAFF85B0D4007622|yoaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGTCTCCTCCTTAG, downstream forward: _UP4_TAATTAGAAAGCGCCCTGTT

    • References

  • 12107147,18763711,10094622,18039762