lonA

class III heat-shock ATP-dependent protease

Locus: BSU_28200

Isoelectric point: 5.77

Molecular weight: 86.42 kDa

Protein length: 774 aa

Gene length: 2325 bp

Function: protein quality control, control of swarming motility

Product: class III heat-shock ATP-dependent protease

Essential: no

Synonyms: lon

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis
• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Additional chemotaxis signal transduction and regulatory proteins

Gene

Coordinates: 2,880,466 → 2,882,790

Phenotypes of a mutant

• presence of predifferentiated swarmer cells in liquid medium PubMed

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of proteins in presence of ATP (according to UniProt)
• inhibits swarming motility by preventing accumulation of SwrA in liquid medium PubMed

Protein family

• Peptidase S16 family (with DdcP and LonB, according to UniProt)

Domains

• Lon N-terminal (aa 9-200) (according to UniProt)
• Lon proteolytic (aa 590-771) (according to UniProt)

Structure

• 6U5Z (from E. coli, 56% identity)
• 5E7S (active hexamer from Meiothermus taiwanensis, 63% identity, 89% similarity) PubMed
• 4YPN (ADP-bound hexamer from Meiothermus taiwanensis, 63% identity, 89% similarity) PubMed
• 3M65 (N-terminal domain) PubMed
• 3M6A (C-terminal domain) PubMed
• 1X37 (Ssd domain)

Localization

• coincident with the nucleoid during normal growth and localized to the forespore during development PubMed

Expression and Regulation

Operons

• Genes: lonA-ysxC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CtsR: repression, PubMed, in CtsR regulon

  Regulation

  • induced by heat stress (CtsR) PubMed

Biological materials

Mutant

• BKE28200 (ΔlonA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGACTGACACCTCCGT, downstream forward: _UP4_AAATGAAAGTCACAAAGTCA
• BKK28200 (ΔlonA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGACTGACACCTCCGT, downstream forward: _UP4_AAATGAAAGTCACAAAGTCA

References

Reviews

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Original publications

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