ansA [2018-12-07 18:03:57]

L-asparaginase

locus: BSU23580

pI: 4.45

mw: 36.30 kDa

protein length: 329 aa

gene length: 987 bp

function: asparagine degradation

product: L-asparaginase

essential: no

ec: 3.5.1.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.6|Utilization of asparagine/ aspartate]

Gene

Coordinates: 2,455,819 → 2,456,808

The protein

Catalyzed reaction/ biological activity

• L-asparagine + H₂O = L-aspartate + NH₃ (according to Swiss-Prot)

Protein family

• asparaginase 1 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|AnsZ]

Structure

• [PDB|5OT0] (from Thermococcus kodakarensis, 43% identity)

Expression and Regulation

Operons

• genes: [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]
  description: [Pubmed|1711029]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1711029], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|146947C974D62BCB282BA3F5B924B35695D75886|AnsR]: repression, [Pubmed|11914346], in [regulon|146947C974D62BCB282BA3F5B924B35695D75886|AnsR regulon]

  regulation

  • expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]

• genes: [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
  description: [Pubmed|22383849]

  regulation

  • ''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]

Biological materials

Mutant

• GP1153 (del ''ansAB''::''ermC'') available in the [SW|Stülke] lab
• BKE23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC
• BKK23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC

lacZ fusion

• pGP872 (in [protein|search|pAC5]), available in [SW|Stülke] lab

References

• 8478318,1711029,11914346,22383849