ansA [2018-12-07 18:03:57]

L-asparaginase

Locus: BSU23580

Isoelectric point: 4.45

Molecular weight: 36.30 kDa

Protein length: 329 aa

Gene length: 987 bp

Function: asparagine degradation

Product: L-asparaginase

Essential: no

E.C.: 3.5.1.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of asparagine/ aspartate

Gene

Coordinates: 2,455,819 → 2,456,808

The protein

Catalyzed reaction/ biological activity

• L-asparagine + H₂O = L-aspartate + NH₃ (according to Swiss-Prot)

Protein family

• asparaginase 1 family (according to Swiss-Prot)

Paralogous protein(s)

• AnsZ

Structure

• 5OT0 (from Thermococcus kodakarensis, 43% identity)

Expression and Regulation

Operons

• Genes: ansA-ansB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AnsR: repression, PubMed, in AnsR regulon

  Regulation

  • expressed in the presence of asparagine (AnsR) PubMed

• Genes: ansA-ansB-mleN-mleA
  Description: PubMed

  Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed

Biological materials

Mutant

• GP1153 (del ansAB::ermC) available in the Stülke lab
• BKE23580 (ΔansA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC
• BKK23580 (ΔansA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC

LacZ fusion

• pGP872 (in pAC5), available in Stülke lab

References

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