function
lactate utilization
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids][SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources]This gene is a member of the following [SW|regulons]
[SW|FbpB regulon], [SW|FsrA regulon], [SW|LutR regulon], [SW|SinR regulon]Gene
Coordinates on the chromosome (coding sequence)
3,494,985 -> 3,495,701
Phenotypes of a mutant
no growth with lactate as the single carbon source [Pubmed|19201793]The protein
Catalyzed reaction/ biological activity
oxidation of lactate to pyruvate [Pubmed|19201793]Expression and Regulation
Operon
''[protein|search|lutA]-[protein|search|lutB]-[protein|search|lutC]'' [Pubmed|19201793][SW|Sigma factor]
[protein|search|SigA] [Pubmed|25031425]Regulation
induction by lactate [Pubmed|19201793]repressed by [protein|search|SinR] [Pubmed|19201793]part of the iron sparing response, down-regulation in a'' [protein|search|fur]'' mutant ([protein|search|Fur], [protein|search|FsrA], [protein|search|FpbB]) [Pubmed|22427629]Regulatory mechanism
dual repression by [protein|search|LutR] (inducer lactate) and [protein|search|SinR] [Pubmed|19201793][protein|search|SinR]: transcription repression [Pubmed|19201793][protein|search|FsrA]: translational repression [Pubmed|22427629][protein|search|FbpB]: acts as RNA chaperone, increases interaction between ''[protein|search|fsrA]'' and ''[protein|search|lutA]-[protein|search|lutB]-[protein|search|lutC]'' RNAs [Pubmed|22427629]Biological materials
lacZ fusion
GP1612 amyE::p(lutA-lacZ cat), constructed with pGP2149 based on [protein|search|pAC5], available in [SW| Jörg Stülke]'s labLabs working on this gene/protein
[SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]References
22427629,16430695,19201793,25031425 
