cimH

transporter for citrate (proton symport)

locus: BSU_38770

pI: 6.55

mw: 48.18 kDa

protein length: 450 aa

gene length: 1353 bp

function: citrate uptake

product: transporter for citrate (proton symport)

essential: no

synonyms: yxkJ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,979,753 → 3,981,105

The protein

Catalyzed reaction/ biological activity

• uptake of citrate (symport with protons) [Pubmed|11566984]

Protein family

• sodium:citrate (SCF) symporter family (together with [protein|F8F62635AA87E4E3A255D6F8FC621EF40954B520|MaeN], according to UniProt)

Paralogous protein(s)

• [protein|F8F62635AA87E4E3A255D6F8FC621EF40954B520|MaeN]

Structure

• [PDB|5X9R] (from Klebsiella pneumoniae, 34% identity) [pubmed|28566738]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]
  description: [Pubmed|21815947]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|10666464]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B744 (yxkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1743 NBRP B. subtilis, Japan]
• BKE38770 (Δ[gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT, downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC
• BKK38770 (Δ[gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT, downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC

References

Reviews

• 16339740

Original publications

• 12525174,10666464,11566984,21815947,28566738